Fig. 4
From: Further identification of a 140bp sequence from amid intron 9 of human FMR1 gene as a new exon
Overexpression of the truncated FMRP protein altered FXS-related signaling pathways. a Partial qRT-PCR results of differentially expressed genes. Red: HEK293T cells transfected by pLEX-MCS-tFMR1; Green: HEK293T cells transfected by pLEX-MCS. b Western blots showing the overexpression of BEX1 protein in HEK293T cells transfected by pLEX-MCS-tFMR1. 1: untransfected HEK293T cells; 2: HEK293T cells transfected by pLEX-MCS-tFMR1; 3: HEK293T cells transfected by pLEX-MCS. c Immunofluorescence showing the localization of BEX1 protein in HEK293T cells. HEK293T: untransfected HEK293T cells; pLEX-MCS-tFMR1: HEK293T cells transfected by pLEX-MCS-tFMR1. DAPI: the solution suitable for nuclear staining; Anti-FMRP: the primary antibodies of FMRP; Anti-BEX1: the primary antibodies of BEX1. d Quantitative representation of FMRP and BEX1 positive cells. **p < 0.01; ***p < 0.001