Table 4 Composition of reaction mixture
From: Genotypes diversity of env gene of Bovine leukemia virus in Western Siberia
Mixture components | Necessary amount, mcl | |
|---|---|---|
Reaction1 | Reaction2 | |
10хof optimized DNA-buffer | 5.0 | 5.0 |
10 mM MgCl2 | 1.0 | 1.0 |
10 mM dNTP | 1.0 | 1.0 |
10 mM primerAP (direct) | 1.0 | 1.0 |
10 mM primerZM2 (reverse) | 1.0 | 1.0 |
2 U/μLTAGpol | 1.0 | 1.0 |
Water, ml | 13.0 | 10.0 |
DNA, 50 ng | 2.0 | 2.0 |