Fig. 3From: The molecular effect of a polymorphic microRNA binding site of Wolfram syndrome 1 gene in dogsGenotyping of rs852850348 SNP by restriction digestion of PCR amplicons. Panel a shows the principle of the applied genotyping method. Amplicons of A allele were digested with the PvuII enzyme into three fragments with different lengths, while in case of G allele only 2 fragments were generated. Panel b shows the separated fragments of various genotypes as detected by horizontal agarose gel electrophoresisBack to article page