Skip to main content

Table 1 Sequencing primers and annealing temperatures used for PCR amplification of dog WFS1 gene regions

From: The molecular effect of a polymorphic microRNA binding site of Wolfram syndrome 1 gene in dogs

Sequencing primers

Forward primer

Reverse primer

Product lenght (base pairs)

TA (°C)

Primer pair 1

CACCCCTGCCACATCCGCAA

CGGCCACGTCGACTCCCAAC

525

59

Primer pair 2

GTCCTGCAGCCCCGGCAATG

GCGACCAGAGGTGTCCGCAG

531

59

Primer pair 3

GGCCGCGGAGGAGCTATGC

CCACGTCGACTCCCAACGCT

562

60

Primer pair 4

ACGGCCGCGGAGGAGCTATG

GTTCCTGTGGTGCTGGTGCCC

537

60

Primer pair 5

CGGAGGAGCTATGCCGCCTGA

GCTGCAGGCAGTTCCTGTGGT

540

60