Fig. 1

Copy numbers of P-element ends in different fly lines as measured by qPCR. One copy of P-element ends corresponds to a single transgene in the heterozygous flies. As expected, no P-element ends were detected in yw; Kr^If-1/CyO; TM3, Sb¹/TM6, Tb¹ flies. In the BDSC lines #8760, #8765, #458, #7415, and #1774, all chromosomes except those carrying P-element insertions (based on the annotations of these lines available at BDSC website) were replaced by chromosomes from the line yw; Kr^If-1/CyO; TM3, Sb¹/TM6, Tb¹ before qPCR analysis: #8760 (yw; Kr^If-1/CyO; Chr3^#8760/TM3, Sb¹); #8765 (yw; Chr2^#8765/Kr^If-1; TM3, Sb¹/TM6, Tb¹); #458 (ChrX^#458/yw; Kr^If-1/CyO; TM3, Sb¹/TM6, Tb¹); #7415 (yw; Kr^If-1/CyO; Chr3^#7415/TM3, Sb¹); #1774 (yw; Kr^If-1/CyO; Chr3^#1774/TM3, Sb¹). In line #1774, two P-element transgenes were detected. The experiment was done in three replicates. Error bars represent standard errors