Fig. 3

Comparison of GFP expression patterns elicited by the elav-Gal4 drivers of the BDSC lines #8760, #8765, and #458 with the Elav immunostaining pattern in the CNS and eye-antennal imaginal discs from third-instar larvae. Maximum intensity projections of confocal images of tissues stained with anti-Elav antibodies and showing GFP expression in the CNS and eye-antennal discs driven by Chr3^#8760 (a-c), Chr3^#8765 (d-f) or Chr3^#458 (g-i). a, d, g The GFP and Elav patterns overlap only partially in the central brain and optic lobes. b, e Colocalization of the GFP and Elav signals in the VNC. Note the ectopic GFP expression in the neuropil (arrows) and in nerve roots (arrowheads). c, f In the eye imaginal discs, the pattern of the GFP expression is narrower compared to anti-Elav antibody staining. h Substantial colocalization of the GFP and Elav signals in the VNC. i In the eye imaginal discs, the patterns of GFP and Elav expression seem largely overlap. CB, central brain; OL, optic lobe; ED, eye imaginal disc; VNC, ventral nerve cord. Scale bars: a, d, g, 200 μm; b, c, e, f, h, i, 100 μm