Fig. 5

Comparison of GFP expression patterns elicited by the 69B-Gal4 driver from the BDSC line #1774 with the Elav immunostaining pattern in the CNS and eye-antennal imaginal discs from third-instar larvae. Maximum intensity projections of confocal images of tissues stained with anti-Elav antibodies are shown. a-d Overlapping of the GFP expression pattern and the Elav localization in the CNS and eye imaginal discs. e-h In the eye-antennal disc, the Elav staining is largely coincident with the GFP signal, but the GFP expression is much broader than that of the Elav protein and includes the anterior area of the eye disc and the antennal disc. i-l Partial colocalization of the GFP and Elav expression patterns in the VNC. m-p Partial overlap between the GFP and Elav signals in the central brain and optic lobes. Note that GFP is expressed only in subset of Elav-positive cells in the VNC. CB, central brain; OL, optic lobe; ED, eye imaginal disc; a, anterior part of the disc; p, posterior part of the disc; VNC, ventral nerve cord. Scale bars: a-d, 200 μm; e-p, 100 μm