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Table 1 Transcriptome analysis of candidate reference genes

From: Optimal reference genes for gene expression analysis in polyploid of Cyprinus carpio and Carassius auratus

Gene

Tissues

Cultured cells

Tissues (FPKM)

log2FoldChange

P value

Cells (FPKM)

log2FoldChange

P value

 

2 N

3 N

JL4N

3 N/2 N

JL4N/2 N

3 N/2 N

JL4N/2 N

2 N

JL4N

SP4N

JL4N/2 N

SP4N/2 N

JL4N/2 N

SP4N/2 N

RPS5

849.24

678.32

473.93

−0.32

− 0.84

0.10

0.01

849.24

856.28

667.33

0.01

−0.35

0.93

0.05

RPS18

2068.00

1038.73

1183.93

−0.99

− 0.80

0.32

0.31

1219.08

1758.57

974.64

0.53

−0.32

0.04

0.22

RPL7

911.01

862.83

224.46

−0.08

−2.02

0.68

0.00

911.01

1107.82

754.63

0.28

−0.27

0.14

0.10

RPLP2

238.01

372.57

949.56

0.65

2.00

0.23

0.14

238.01

442.26

153.76

0.89

−0.63

0.00

0.10

RPL13α

982.73

715.70

251.13

−0.46

−1.97

0.15

0.00

1083.97

1434.20

689.13

0.40

−0.65

0.01

0.02

β-actin

578.00

128.05

435.73

−2.17

−0.41

0.14

0.57

1212.30

2115.03

1517.57

0.80

0.32

0.08

0.33

EF1-α

2074.67

2286.30

855.60

0.14

−1.28

0.65

0.02

3693.40

4006.77

1957.93

0.12

−0.92

0.79

0.17

DDX5

68.30

88.85

41.80

0.38

−0.71

0.23

0.26

74.10

90.77

80.90

0.29

0.13

0.08

0.35

β-tubulin

29.80

51.30

33.67

0.78

0.18

0.09

0.71

240.73

273.17

294.77

0.18

0.29

0.64

0.45

hprt1

26.93

47.40

20.93

0.82

−0.36

0.27

0.64

28.27

15.53

19.10

−0.86

−0.57

0.33

0.47

B2M

96.53

263.95

89.73

1.45

−0.11

0.02

0.91

200.10

100.97

95.13

−0.99

−1.07

0.02

0.02

GAPDH

2324.47

1332.75

539.83

−0.80

−2.11

0.32

0.04

0.70

0.93

0.67

0.41

−0.06

0.63

0.93