Fig. 5
From: miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4
miR-34a inhibits lipogenesis in porcine preadpocytes. a Detection of miR-34a mimic after transfection for 24, 48, and 72 h. mimic nc: mimic negative control; nc: blank negative control. b Expression of mRNA from genes related to lipogenesis and steatolysis in differentiated cells transfected with miR-34a mimic, mimic negative control, inhibitor, inhibitor negative control, and blank negative control. c Proteins detected by western blotting in the mimic, inhibitor, and nc groups. d Protein expression in the mimic, inhibitor, and nc groups. e Oil Red O staining of terminally differentiated adipocytes. f TG levels in the mimic, inhibitor, and nc groups. Results are presented as means ± SE; n = 3; ** P < 0.01. Labels (a, b, c) indicate significantly different values (P < 0.05)