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Table 1 Checklist for experimentally validated miRNA-target interaction (MTI)

From: miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4

Item

Results

1. miRNA gene name (Entrez ID)

miR-34a (100316602)

2. Target gene name (Entrez ID)

ACSL4 (448980)

3. Species name (Species ID)

Sus scrofa domesticus (9825)

4. Genomic location of MTI Nucleotide sequence

5'CACTGCC

Location according to Ensembl

X:89761656-89761650

Location within a part of a gene

1934-1940 (location within 3'UTR)

Method for experimental validation

Luciferase reporter assay, qPCR, western blot

Cell lines

293 T cell lines, porcine primary intramuscular preadipocytes

5. Sequence variants within MTI

None

6. Associated phenotype

Adipogenesis

  1. Note:Checklist was prepared according to guidelines for the miRNA target reporting standardization [55]