Table 1 Adapters and primers used in the amplification of genomic DNA.
From: SNP identification, verification, and utility for population genetics in a non-model genus
Adapters | |
|---|---|
BspEI (5' to 3') | |
GACGATGAGTCCTGAGC | |
CTGCTACTCTCAGGACTCGGGCC | |
EcoRI (5' to 3') | |
CTAGAGTCCTAGTAGCACCTCGTAGACTGCGTACC *CATCTGACGCATGGTTAA | |
Preselective Primers | |
EcoRI (5' to 3') | |
CTGAGTCCTAGTAGCACC | |
BspEI (5' to 3') | |
GACGATGAGTCCTGAGC | |
Selective Primers | |
EcoRI (5' to 3') | |
GACTGCGTACCAATTCAAG | |
BspEI (5' to 3') | |
GACGATGAGTCCTGAGCC | |
Barcoded Primers | |
EcoRI (5' to 3') | |
1 | GCCTCCCTCGCGCCATCAGAGCCTAAGCTGACTGCGTACCAATTCAAG |
2 | GCCTCCCTCGCGCCATCAGAGTTCAAGTCGACTGCGTACCAATTCAAG |
3 | GCCTCCCTCGCGCCATCAGACTTGAACTGGACTGCGTACCAATTCAAG |
4 | GCCTCCCTCGCGCCATCAGACGGTAACGTGACTGCGTACCAATTCAAG |
5 | GCCTCCCTCGCGCCATCAGATCCGAATCGGACTGCGTACCAATTCAAG |
6 | GCCTCCCTCGCGCCATCAGATGGCAATGCGACTGCGTACCAATTCAAG |
7 | GCCTCCCTCGCGCCATCAGCAGGTCCAGTGACTGCGTACCAATTCAAG |
8 | GCCTCCCTCGCGCCATCAGCATTGCCATGGACTGCGTACCAATTCAAG |
9 | GCCTCCCTCGCGCCATCAGCTAAGCCTAGGACTGCGTACCAATTCAAG |
10 | GCCTCCCTCGCGCCATCAGCGAATCCGATGACTGCGTACCAATTCAAG |
BspEI (5' to 3') | |
GCCTTGCCAGCCCGCTCAGGACGATGAGTCCTGAGCC | |