| Forward sequence | Reverse sequence | Tm | Size |
---|---|---|---|---|
Nle1 sequencing primers | ||||
Nle1E1/2 | CTTGACTCCTCCGAACACGAG | AAACACAGCCTGTCTGTAGGTGAG | 62 | 500 |
Nle1E3 | GATTAAATTTGTCGCATGGTGGTA | GTCTGTTACTTGCAACGTGAGTCC | 62 | 475 |
Nle1E4 | TATTTCTCCTCAGGGAATGGAGAG | CCACACTCAGTCCAGTATCTGCTT | 62 | 377 |
Nle1E5/6 | CTGTGTTCTCCCTCACCTCTCC | ATAGTAGGCCAAGCCGTTGCT | 62 | 557 |
Nle1E7 | ACAGCCTTGCTCTGCTGTTAGAA | GGACCAGCTGGACTCTTGGTATAA | 62 | 440 |
Nle1E8/9 | TTCCTGATTCTTGCCTTATGTCAC | AACCCTAACTAAGACAACCAAGAACAA | 62 | 544 |
Nle1E10 | TGGAGTTGCATGTAAGCTTGTGT | GTCACTAGCCCTAAAGATGCCATT | 62 | 488 |
Nle1E11/12 | CCGGCCCAGGTACCTAGCTT | ACCTACAGGTTCTCCCAGAGTCTCC | 62 | 498 |
Nle1E13 | ACTTGATACTTGGCAGTAGGCACA | CTCCTGCTATCCAGTGCAAGG | 62 | 570 |
Nle1 genotyping primers | ||||
Nle1 GSP | GCTGTAATGTCCTGACTGT | Â | 60 | 637 |
cDNA 1 | CTGTGTCGTACTCTTCAAGGTCAT | CTGTGGAGTCATCTTCTCCATATC | Â | Â |
cDNA 2 | TCAGACGACTTCACCTTATTCCTG | CAGTCAACAGCATATACCTCATCG | 62 | 351 |
Nle1 DNA | TCTCCTTCAGCTCCTTCACTGT | TCCAATGGTGGAGTATAGGGTATAA | 60 | 341 |
cDNA amplification primers | ||||
1st PCR | ATATGGATCCGGCGCGCCGTCGACT24 | ATATCTCGAGGGCGCGCCGGATCCT24 | 67 | Â |
2nd PCR | (NH2)ATATGGATCCGGCGCGCCGTCGACT24 | (NH2)ATATCTCGAGGGCGCGCCGGATCCT24 | 67 | Â |
Eed | GTGTGACATTTGGTACATGAGGTT | ACATTTATGATGGGTCAGTGTTGT | 60 | 148 |
TaqMan gene expression assay | ||||
 | Assay ID | Amplicon Size (bp) |  |  |
Trp53 | Mm01731290_g1 | 119 | Â | Â |
Cdkn1a | Mm04205640_g1 | 80 | Â | Â |
Gapdh | Mm99999915_g1 | 107 | Â | Â |