Table 1 List of 25 loci along with the primer pairs
From: Development of a model webserver for breed identification using microsatellite DNA marker
Locus | Forward primer | Reverse primer | Dye | Size range | No. of observed allele |
|---|---|---|---|---|---|
ILST008 | gaatcatggattttctgggg | tagcagtgagtgaggttggc | FAM | 167–195 | 12 |
ILSTS059 | gctgaacaatgtgatatgttcagg | gggacaatactgtcttagatgctgc | FAM | 105–135 | 14 |
ETH225 | gatcaccttgccactatttcct | acatgacagccaagctgctact | VIC | 146–160 | 9 |
ILST044 | agtcacccaaaagtaactgg | acatgttgtattccaagtgc | NED | 145–177 | 16 |
ILSTS002 | tctatacacatgtgctgtgc | cttaggggtgtattccaagtgc | VIC | 113–135 | 14 |
OarFCB304 | ccctaggagctttcaataaagaatcgg | cgctgctgtcaactgggtcaggg | FAM | 119–169 | 31 |
OarFCB48 | gagttagtacaaggatgacaagaggcac | gactctagaggatcgcaaagaaccag | VIC | 149–181 | 21 |
OarHH64 | cgttccctcactatggaaagttatatatgc | cactctattgtaagaatttgaatgagagc | PET | 120–138 | 10 |
OarJMP29 | gtatacacgtggacaccgctttgtac | gaagtggcaagattcagaggggaag | NED | 120–140 | 14 |
ILSTS005 | ggaagcaatgaaatctatagcc | tgttctgtgagtttgtaagc | VIC | 174–190 | 9 |
ILSTS019 | aagggacctcatgtagaagc | acttttggaccctgtagtgc | FAM | 142–162 | 11 |
OMHC1 | atctggtgggctacagtccatg | gcaatgctttctaaattctgaggaa | NED | 179–209 | 27 |
ILSTS087 | agcagacatgatgactcagc | ctgcctcttttcttgagagc | NED | 142–164 | 11 |
ILSTS30 | ctgcagttctgcatatgtgg | cttagacaacaggggtttgg | FAM | 159–179 | 12 |
ILSTS34 | aagggtctaagtccactggc | gacctggtttagcagagagc | VIC | 153–185 | 15 |
ILSTS033 | tattagagtggctcagtgcc | atgcagacagttttagaggg | PET | 151–187 | 25 |
ILSTS049 | caattttcttgtctctcccc | gctgaatcttgtcaaacagg | NED | 160–184 | 13 |
ILSTS065 | gctgcaaagagttgaacacc | aactattacaggaggctccc | PET | 105–135 | 16 |
ILSTSO58 | gccttactaccatttccagc | catcctgactttggctgtgg | PET | 136–188 | 27 |
ILSTSO29 | tgttttgatggaacacagcc | tggatttagaccagggttgg | PET | 148–191 | 23 |
RM088 | gatcctcttctgggaaaaagagac | cctgttgaagtgaaccttcagaa | FAM | 109–147 | 19 |
ILSTS022 | agtctgaaggcctgagaacc | cttacagtccttggggttgc | PET | 186–202 | 9 |
OARE129 | aatccagtgtgtgaaagactaatccag | gtagatcaagatatagaatatttttcaacacc | FAM | 130–175 | 23 |
ILSTS082 | ttcgttcctcatagtgctgg | agaggattacaccaatcacc | PET | 100–136 | 19 |
RM4 | cagcaaaatatcagcaaacct | ccacctgggaaggccttta | NED | 104–127 | 12 |