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Fig. 2 | BMC Genetics

Fig. 2

From: Multiple regulatory variants located in cell type-specific enhancers within the PKP2 locus form major risk and protective haplotypes for canine atopic dermatitis in German shepherd dogs

Fig. 2

Regulatory potential and allelic differences of candidate regions. Luciferase reporter assays for each of the eight evaluated SNPs (SNP 27:18,861,228, SNP 27:19,086,778, SNP 27:19,093,355, SNP 27:19,096,199, SNP 27:19,112,169, SNP 27:19,114,170, SNP 27:19,135,677, and SNP 27:19,140,837) were performed in four different cell lines: a. MDCK, b. HaCaT, c. Jurkat, and d. K562. The cloned fragments showed differential activity in different cell lines, indicating the presence of cell type-specific enhancers. The fragment with the SNP 27:19,086,778 contained the most potent enhancer showing the highest activity in HaCaT cells (8 vs. 11-fold induction) with significant allelic difference (p = 0.003). All except SNP 27:19,114,170 showed allelic differences in at least one cell line

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BMC Genomic Data

ISSN: 2730-6844

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