Table 4 Primers used in this study
Gene | Sequence (5'-3') | Amplicon length (bp) | Annealing temperature (°C) |
|---|---|---|---|
IS900- MAP (external) | F-AGGGTGTTCGGGGCCGTCGCTTAG R-TGAGGTCGATCGCCCACGTGACCT | 406 | 56.5 |
IS900-MAP (internal) | F-ATGTGGTTGCTGTGTTGGATGG R-CCGCCGCAATCAACTCCAG | 298 | 63.0 |
TLR1 | F-GGAGATACTTATGGGGAAAGAGAA R-GTGTATAGACAAGGCCTTCAGTGA | 402 | 52.0 |
TLR2 | F-CAGGAGCTGGAGCACTTGTACC R-GTCTCATCCACGGGCCAGACCA | 362 | 56.0 |
TLR4 | F-GGGACTGTGCAACCTGACCA R-GCTCTAAGCCCATGAAGTTTGAA | 434 | 53.0 |
IL-4 | F-CCCAGCGCTGGTCTGCTTACT R-GCTTGCCAGGCTGCTGAGATT | 283 | 57.4 |
IL-8 | F-TTGGCCGCTTTCCTGCTCT R-AAATGCCTGCACAACCTTCTGC | 249 | 55.2 |
IL-10 | F-AGCCGAGATGCCAGCACCCTGTC R-AGCTTCTCCCCCAGCGAGTTCACG | 293 | 61.0 |
IL-12p35 | F-GAGCCTGCCCACCACCACA R-GGAAGCCAGGCAACTCTCATT | 226 | 56.4 |
IFN-γ | F-CTAAGGGTGGGCCTCTTTTCTC R-CATCCACCGGAATTTGAATCAG | 237 | 53.2 |
β-actin | F-ACTGGGACGACATGGAGAG R-AGGAAGGAAGGCTGGAAGAG | 568 | 54.0 |