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Figure 3 | BMC Genetics

Figure 3

From: A modifier screen in the Drosophila eye reveals that aPKC interacts with Glued during central synapse formation

Figure 3

Mutations that interact with Glued and alter GF axon morphology. (A) Schematic of the adult CNS with the GFs indicated. Hatched box indicates the approximate area of the ventral ganglia depicted in B-D & F-K. (B-K) Dissected adult nervous systems stained for LacZ expression. (B) UAS-LacZ; A307 control showing normal GFs with their characteristic bends in the mesothoracic neuromere (arrowhead) where the GF synapses with the TTMn. (C) A fly also expressing UAS-GlDN exhibits swollen, bendless, axon tips (asterisk). (D) The introduction of EG37/+ did not noticeably enhance the swollen axon phenotype. (E) The whole nervous system is shown for this preparation in which the introduction of EG28/+ results in a more severe phenotype with one GF remaining in the brain. (F-H) Three different suppressors showing amelioration of the swollen axon phenotype. Note that GFs from specimens carrying SG13/+ or SG46/+ show normal diameter axons and sometimes a terminal bend (arrowhead) and that the GFs from specimens carrying SG58/+ also exhibit normal diameter axons. (I) A fly carrying the Gl1 mutation exhibits wild type GF morphology. (J&K) preparations from flies carrying Gl1 and an enhancer sometimes showed enhancement or altered GF phenotypes (see Table 1 and text for details). Scale bar is 5 μm.

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