Skip to main content

Table 4 Summaries of EST-SSR primers and repeat motif.

From: Analysis of expressed sequence tags from Prunus mume flower and fruit and development of simple sequence repeat markers

Primer

Repeat motif

Primer sequence (5'→3')*

Tm(°C)

Expected product (bp)

ES1

(AG)10

F: CTCTTGTTTCTCCTCCTACT

57.6

253

  

R: GACGAGTTTATCGTTGACT

  

ES2

(TG)13+(TC)20

F: TTGAGGTTGAGCCTACAC

56

306

  

R: GCAAGGTCACTATCTTTTTC

  

ES3

(TGG)7+(TGGTGT)3

F: GCTATACACGCTGCTAATA

54.6

259

  

R: AAGGCATCACATCAACAC

  

ES4

(AGC)7

F: CCGTAATAACCACCGTCC

58.2

240

  

R: CCGCCTTCATCATCCTCT

  

ES5

(CAA)8

F: CCAGATCCACTATTTCTTC

55.9

267

  

R: GTGTTAGAGCCAGAAACC

  

ES6

(AGTG)5

F: GCACTCTTCTCTCTCTCTCT

56.5

312

  

R: GAGACCTTATGGAAGAAAAC

  

ES7

(TTTTG)4

F: GAGAGAGACAAACAAGTGAA

55.5

253

  

R: CTTGAGGAGTGATTTCCTA

  

ES8

(TC)23

F: ACAGTTTCAGAATCTCACAG

57.6

284

  

R: GATGGGACTTAAGAAGAGTC

  

ES9

(AG)15

F: CCCTCTTATTCTCTCTCACT

56.5

207

  

R: CTTCAATATACTTGGTGAGC

  

ES10

(GA)22

F: CTACGTACTTCCTGAGTGAG

56.5

220

  

R: CTAAAGATCGTTCAGACTGT

  

ES11

(GTCT)3(GA)13

F: GGGTGTTGTGTCTGTTGGA

58.7

287

  

R: ACGAGGAAGATGAGGAGGG

  

ES12

(TTG)7

F: TTCCTGCTATCTGCTCCAAT

58.7

344

  

R: GTGACGATGCTGTGCTCTGT

  

ES13

(AT)10

F: GAGGAAATATTCCTGCATCA

55.5

188

  

R: CTGTTTCGTCATCTTTTTCC

  

ES14

(AGA)7(GGA)4

F: ACATATCCACCACCACCAAC

58.6

254

  

R: AAAACAGAACACGACCCAGA

  
  1. * Every two primers belong to one pair. F is the forward and R is the reverse primer.
Back to article page

BMC Genomic Data

ISSN: 2730-6844

Contact us