Table 1 Oligonucleotides
Oligonucleotide combination | Oligonucleotide forward (5'-3') | Oligonucleotide reverse (5'-3') | Size (bp) |
|---|---|---|---|
1: Intron 3 | AGCGTCTTCAGAAGCAGAAC | CCACATCAGCTCTCCTACAG | 350 |
2: Intron 4 | CTACGACAGCAAGCTGGTGGACTT | GGTCAAGCCGTTCCATGAGTTCAC | 600-700 |
3: Intron 5 | CTCCCCAGTGTAACATGATCAACGG | CAGATTGTCTGCAGGGACAGAAGC | 600 |
4: Intron 10 | GGGAAGATCTCAGAGGTTGT | TACACCATCACCATTGGCAA | 500 |
5: Exon 4 and 5 | CTACGACAGCAAGCTGGTGGACTT | GATGTCATCTTCGCCGGTATGGATT | 138 |
6: cDNA | CGTTGGACCAATCATTATCGACA | TCTGTAGTGTAGTAGTACAGCTCCCAGAG | 1308 |
7: cDNA | ATGAATAAATCTAAATTAGCGATCGGA | TGTCATCTTCGCCGGTATGGATT | 672 |
8: cDNA | TGATGGAGAACTTGCCGTTCC | CCCTGCAGCACTGGGCTAGACT | 1054 |