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Table 1 Strains used in this study

From: Functional characterization of the Saccharomyces cerevisiae protein Chl1 reveals the role of sister chromatid cohesion in the maintenance of spindle length during S-phase arrest

Strain Genotype Sources/References
AP22 MAT α leu2-3,112 his3-11,15 ura3-52 trp1 [36]
AP22Dchl1 MAT α leu2-3,112 his3-11,15 ura3-52 trp1 chl1::HIS3 [36]
8534-8C MAT α his4Δ34 ura3-52 leu2-3,112 [69]
8534-10A MAT a his4Δ34 ura3-52 leu2-3,112 [73]
301-2B MATα leu2-3,112 hisΔ34 ura3-52 trp1 [66]
PS29-2B MAT a leu2-3,112 his3-11,15 mcm18-1/ctf19 [69, 74]a
M29-5D MAT a leu2-3,112 hisΔ34 ura3-52 trp1mcm18-1/ctf19 This study, by crossing 301-2B with PS29-2B
A3 MAT a leu2-3,112 his3-11,15 [69]
699 MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100 [37]
699Matα MATα ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100 This study
699Dchl1 MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100 chl1::HIS3 [37]
SL14 MAT a ade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 bar1Δ::LEU2 [37]
SL14Dchl1 MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100 bar1Δ::LEU2 chl1::HIS3 [37]
US354 MATα leu2 his3 trp1 ade2 ura3 rad53-21 [37]
SL7 MAT a leu2 his3 trp1 ade2 ura3 rad53-21 [37]
US3329 MAT a leu2::LEU2::tetR-GFP trp1 CEN5::tetOX224::HIS3 ade2-1 ura3 his3 [75]
US3324 MAT a ade2-1 leu2-3,112 his 3-11,15 can1-100 scc1-73 Uttam Surana
SL20 MATα ade2-1 can1-100 leu2-3,112 his3-11,15 scc1-73 This study, by crossing US3324 with 699Matα
SL25 MAT a leu2::LEU2 tetR-GFP ade2-1 CEN5::tetOX224::HIS3 ura3 scc1-73 This study, by crossing US3329 with SL20
US3329Δchl1 MAT a leu2::LEU2:: tetR-GFP ura3 CEN5::tetO X224::HIS3 ade2-1 chl1Δ::TRP1 This study, by deleting CHL1 in US3329
US3329Δmcm17 MAT a leu2::LEU2:: tetR-GFP ura3 CEN5::tetOX224::HIS3 ade2-1 mcm17Δ::URA3 This study, by deleting MCM17 in US3329
US3329Dmcm21 MAT a leu2::LEU2:: tetR-GFP ura3 CEN5::tetOX224::HIS3 ade2 mcm21::URA3 This study, by disrupting MCM21 in US3329
US3335 MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3-1 can1-100 SCC1-18MYC::TRP1 Uttam Surana
US3335Dchl1 MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3-1 can1-100 SCC1-18MYC::TRP1 chl1::HIS3 This study, by disrupting CHL1 in US3335
US3335Δsir3 MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3-1 can1-100 SCC1-18MYC::TRP1 sir3Δ::HIS3 This study, by deleting SIR3 in US3335
  1. 699 and all the strains listed below it are in W303 background, while the strains listed above 699 were from G. Fink.
  2. a The MCM18 gene is the same as CTF19 [74, Saccharomyces Genome Database http://www.yeastgenome.org]. The PS29-2B strain [69] contains the mcm18-1 allele of the MCM18/CTF19 gene.