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Table 3 Resolution between consecutive steps of the dilution series determined by the P-values of a two-tailed T-test for comparing averages

From: Empirical evaluation of humpback whale telomere length estimates; quality control and factors causing variability in the singleplex and multiplex qPCR methods

Assay Target Batches Exp amount DNA (ng/reaction)
    72.0-36.0 36.0-18.0 18.0-9.0 9.0-4.5
I Tel 7 0.103 0.051 0.013 0.005
  Ref 7 0.029 0.052 0.024 0.022
IV Tel 4 0.014 0.001 0.000 0.002
  Ref 4 0.010 0.009 0.007 0.012
Assay Target Batches Exp amount DNA (ng/reaction)  
    60.0-20.0 20.0-6.7 6.7-2.2  
II Tel 4 0.013 0.007 0.002  
  Ref 4 0.019 0.018 0.254  
III Tel 4 0.000 0.001 0.000  
  Ref 4 0.032 0.002 0.020  
  1. Bold values are non-significant at the 5% level and indicate that there is no difference between the amounts of DNA observed at two consecutive dilution steps.