Table 2 SNP and haplotype pattern of the phenylalanine ammonia-lyase (PAL) encoding gene, assessed by pyrosequencing assays for the SNPs 1, 3, 4 and 5 in a set of 190 European barley cultivars and the 6-rowed mapping parents Steptoe and Morex
Haplotypes | SNPs from 5' to 3' | Haplotype frequency (No. of cultivars) according row number and growth habit | Haplotype frequency (No. of cultivars) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Â | SNP1 | SNP3 | SNP4 | SNP5 | 2r-S | 6r-S | 2r-W | 6r-W | Â |
PAL_H1 | A (Pro) | C (Leu) | A (Arg) | G (Ala) | 80 | – | 38 | 3 | 121 |
PAL_H2 | C (Pro) | C (Leu) | A (Arg) | G (Ala) | 13 | 1 | 3 | 25 | 42 |
PAL_H3 | C (Pro) | T (Leu) | T (Arg) | A (Ala) | 1 | 1 | 20 | 6 | 28 |
Unknown | Missing SNP information | – | – | – | 1 | 1 | |||
Total | Â | Â | Â | Â | 94 | 2 | 61 | 35 | 192 |