Figure 3

LM-PCR evidence that cis-acting sequences determine TAS positions. 3A. Karyonidal reproducibility of CR-Right TAS display patterns. Two display lanes represent CR-R TASs in two karyonides with identical heterozygous 81-locus genotypes, 310/510b. Also shown are two lanes representing two karyonides with 310/510a genotypes. Arrows indicate the positions of distinct allele-specific bands. Specific oligomers used: Extend = ST2; P1 = PEB; Display = VHO. 3B. Allele-specificity of TAS positions, and "verification" of one set of display bands. DNAs from Oxytricha strains with various 81-locus allele compositions were used as templates for LM-PCR and display (see text). Arrows indicate the positions of distinct allele-specific bands. Specific oligomers used: Extend = ST2; P1 = PEB; Display = VHO. A verification PCR reaction was performed with the P1-7A PCR product from O. fallax 3.5, and display lanes are run along side (see text for explanation of this verification procedure). Because short chains ran especially rapidly, the bottoms of the "fal 3.5" lanes are presented to the side of the main panel. Four corresponding bands in the P1-anchor PCR display and the verification display are marked. 3C. Allele-specificity of TAS display pattern for the multi-TAS region bounding the Left end of Common Region. DNAs from strains with various 81 locus genotypes were used as templates for LM-PCR and display (see text). Arrows indicate the positions of distinct allele-specific bands. Specific oligomers used: Extend = PEB-prime; P1 = oRG; P2 primer = T1target; Display = 180L-.