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Table 1 Growth. Wild type, td-Gpm mutant, and ubr1-suppressed mutant were assessed at three temperatures in enriched or minimal medium with 1% glucose.

From: Setting of graded levels of a protein in yeast by a t-degron technique as applied to phosphoglycerate mutase

  Wild type td-Pgm Mutant ubr1-Suppressed Mutant
    24°C 30°C 37°C 24°C 30°C 37°C 24°C 30°C 37°C
Medium R col., 3d mm 1.7 2.8 3 1.4 2.2 0.1 1.5 2.1 3.2
" Mu h-1 ND 0.39 0.39 ND 0.37 0.07 ND 0.41 0.41
" vglucose umol*h-1* ODU-1 ND 2.7 2.9 ND 1.4 ND ND 2.7 2.7
Medium M col., 3d   1.1 1.6 1 0.8 0.7 <0.1 1 1.5 1.1
" (+ Ant A) col., 3d   0.3 0.9 1.1 0.2 <0.1 <0.1 0.3 1 0.8
" (+ Ant A) col., 5d   1 1.7 1.9 0.7 <0.1 <0.1 1 1.5 1.3
  1. ND, not done. Col., average colony size in mm at the indicated time of incubation, using plates spread with not more than 50 cfu. vglucose is the rate of glucose metabolism. Ant A, antimycin A, 2 ug/ml.