Figure 6

Some of the tab mutants show nuclear transport defects. Strains of the indicated genetic background were transformed with a CEN/ARS plasmid expressing Rpl11b-GFP, and grown at 25°C. A portion of the cultures were then shifted to 37°C for 1 hr and shifted back to 25°C for 35 min before the 37°C -> 25°C samples were taken. (A) Rpl11 b-GFP was visualized as described previously [39]. (B) The percentage of cells (+/- 1%) with preferential nuclear Rpl11b-GFP staining in each sample was counted (n ≥ 100).