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Table 3 ncl-1 versus GFP-LacI•lacO as markers for mosaic analysis.

From: Visualization of C. elegans transgenic arrays by GFP

  Phenotype of cells
Cell W, + or N,- W, - N, + ?
m2 32 (87%) 0 3 (8%) 2
m3L 31 (84%) 2 (5%) 4 (11%)  
m3VL 31 (84%) 1 (3%) 5 (13%)  
m4 28 (76%) 0 9 (24%)  
m3R 31 (84%) 2 (5%) 4 (11%)  
m3VR 30 (81%) 1 (3%) 6 (16%)  
m3DR 29 (78%) 0 8 (22%)  
m3DL 30 (81%) 0 7 (19%)  
hyp7(dorsal head) 30 (81%) 1(3%) 6 (16%)  
hyp7 (ventral head) 32 (87%) 0 5 (13%)  
P/I 33 (89%) 1 (23%) 3 (8%)  
P3.p 30 (81%) 1 (3%) 3 (8%) 3
P4.p 29 (78%) 2 (5%) 4 (11%) 2
P5.p 30 (81%) 2 (5%) 4 (11%) 1
P6.p 32 (87%) 0 4 (11%) 1
P7.p 33 (89%) 0 3 (8%) 1
P8.p 26 (70%) 1 (3%) 9 (24%) 1
B 22 (59%) 1 (3%) 14 (38%)  
F 31 (84%) 1 (3%) 5 (13%)  
U 29 (78%) 2 (5%) 6 (16%)  
hyp7 (ventral tail) 29 (78%) 0 7 (19%) 1
hyp7 (anus) 21 (57%) 1 (3%) 8 (22%) 7
Mean % 80% 2% 16% 2%
Standard Dev 8% 2% 7% 4%
  1. Mosaic animals from strain PS3047 (n = 37) were used to determine the occurrence of the Ncl phenotype and GFP-LacI•lacO. Scored by cell from anterior to posterior of the worm: m2, m3L, m3VL, m4, m3R, m3VR, m3DL, m3DR, hyp7(dorsal, head), hyp7(ventral head), P/I, P3.p, P4.p, P5.p, P6.p, P7.p, P8.p, B, F, U, hyp7(tail), hyp7(anus). +: GFP-LacI bound to the lacO, fluorescent spot; -: diffuse fluorescence, W: normal nucleolus (non-Ncl) size, N: enlarged nucleolus (Ncl) and ?: undetermined.