Figure 2From: Hemi-nested touchdown PCR combined with primer-template mismatch PCR for rapid isolation and sequencing of low molecular weight glutenin subunit gene family from a hexaploid wheat BAC libraryPhylogenetic tree of partial LMW-GS gene sequences using primer pair LMWF11/LMWR11 constructed using MEGA3.1. Bootstrap values are indicated; bootstrap percentages are based on 1000 iterations. The scale bar indicates the level of sequence divergence. DNA sequences with the same N-terminal amino acid sequence were clustered in the same group.Back to article page