Multispecies protein sequence alignment of four parts of LRP4. The observed mutations are indicated by arrows and the respective residues are indicated in bold characters. Conserved cysteines (A+D) and YWTD motifs (B+C) are highlighted in grey. Protein sequences accession numbers used for the alignment: Bos taurus (ABE73152), Homo sapiens (NP_002325), Canis familiaris (XP_540748), Mus musculus (NP_766256), Rattus norvegicus (NP_112612), Gallus gallus (XP_421114), Tetraodon nigroviridis (CAF99960), Aedes aegypti (EAT37281), Drosophila melanogaster (NP_727914). Small and hydrophobic amino acids are indicated in red, acidic amino acids in blue, basic amino acids in magenta, and hydroxyl-, amine-, basic-Q amino acids in green, and others in gray, respectively. Identical residues are indicated by asterisks beneath the alignment, while colons and dots represent very similar and similar amino acids, respectively.