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Table 2 Bias and standard deviation (SD) of each method for each duplicated copy ratio. Root mean square (RMS) of the bias and SD is calculated to compare accuracy and precision among the three standard curves.

From: An accurate method for quantifying and analyzing copy number variation in porcine KIT by an oligonucleotide ligation assay

Duplicated copy ratioa (%)

Bias of qOLA_CNV (Height, %)

SD of qOLA_CNV (Height, %)

Bias of qOLA_CNV (Area, %)

SD of qOLA_CNV (Area, %)

Bias of Pyro_CNV (%)

SD of Pyro_CNV (%)

0

0

0

0

0

3.64

0.38

10

1.55

0.51

1.91

0.24

-1.13

1.30

20

3.43

0.42

3.67

0.46

-5.87

0.31

30

4.01

0.56

3.31

0.93

-4.48

0.69

40

3.09

0.31

1.92

1.08

-7.17

1.04

50

2.32

0.58

3.40

1.95

-7.48

1.49

60

1.27

0.61

-1.65

1.32

-5.23

0.97

70

0.51

0.33

-2.07

0.93

-3.75

0.93

80

-0.56

0.72

-2.91

1.34

-1.04

0.92

90

-0.87

0.13

-2.01

1.03

-3.62

1.81

100

0

0

0

0

6.98

0.52

RMS1b

2.09

0.45

2.16

1.03

5.05

1.04

RMS2c

0.85

0.51

2.21

1.17

3.73

1.21

  1. a, The ratios were described as expected ratio of duplicated copy on the x-axis of Figure S2.
  2. b, Overall RMS
  3. c, RMS for the zone between 60 – 90%