Figure 5

Meiotic phenotype of ms(3)168-112 and ms(3)127-109 lines. Left column: immunostaining with anti α-tubulin; right column: Hoechst 33258 staining. For comparison, see also the wild type meiosis reported in Figure 4. a-b: early prometaphase in primary spermatocytes of ms(3)168-112 mutants; chromatin is condensed (b) as in wild type but asters are absent (a). c-d: late prometaphase in ms(3)127-109 primary spermatocytes; arrows in (d) point to aggregated mitochondria resembling nebenkern. e-f: a meiotic cell, from ms(3)127-109 testes showing multiple α-tubulin nucleation foci (e) and four nuclei of different sizes (f). g-h: abnormal spermatids during the elongation stage in ms(3)168-112 males; note the irregular organization of microtubules (g) and the unusual chromatin distribution (h). i: spermatids from the ms(3)168-112 line showing irregular nuclei/nebenkern associations and irregular nuclear size. Bars: 10 μm