Figure 2

Sorbitol partially suppresses slow growth at 37°C due to tra1 _{SRR 3413} . Yeast strains CY2222 (tra1_{SRR 3413}) and BY7092 (TRA1) were grown overnight to saturation in YPD at 30°C. Cells were diluted to approximately 1000 cells per μl and 10 μl of 10-fold serial dilutions spotted on synthetic complete media containing 2% glucose without (SC) or with 1.0 M sorbitol. Cells were grown for 4 days at 37°C.