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Table 1 Summary data of microsatellites La Gomera Giant lizard ( Gallotia bravoana ) for all loci (A) for wild individuals (B) and for captive samples (C)

From: Island survivors: population genetic structure and demography of the critically endangered giant lizard of La Gomera, Gallotia bravoana

A) Loci

 

Locus

Repeat motif

Ta (°C)

Primers sequence 5’-3’

Allele range

N

N A

N AR

H O

H E

F IS 1

GenBank No.

 

GBR5

 

(CA)10

60

F: ATATTCATCCTCCCCGCACA

177

90

1

-

-

-

-

JX661253

F: GCATTGCGGTGAAAAAGG

 

GBR9

 

(GT)17

60

F: TGGAGGCTTCTCTTGAGGCAAGA

138-160

98

4

3.9

0.143

0.153

0.066*

JX661254

F: CCCCCTGCCTTATGAGTTTCG

 

GBR11

 

(GTCT)6 (ATCT)12

60

F: CTTAACCGTCTGGTTTGCATTA

196-215

96

2

2.0

0.000

0.021

1.000*

JX661255

F: ACTGCACCCCATAGTTGTCTTT

 

GBR15

 

(CA)27

60

F: ACTGGGGCTCAGTCTTTGTTT

140

91

1

-

-

-

-

JX661256

F: GCGTGTCTTGTGTATATGGAATC

 

GBR16

 

(CA)14

60

F: GCAGATTTAATGGAACCTGGAG

224-238

91

2

2.0

0.011

0.033

0.664*

JX661257

F: CAACAAAATGTGGAGTTTTAGCC

 

GBR20

 

(GT)16 (GTAA)7

60

F: CCACAACAAAACAAATGCAA

190-217

97

5

4.9

0.051

0.081

0.362*

JX661258

F: GTCAGATCGACCCTCTCAGC

 

GBR24

 

(CA)28

60

F: ACTTGCAGACTATTTTGGGTT

129-167

96

4

3.9

0.531

0.512

-0.039*

JX661259

F: ACTCGCATCCTTCTGTTACAA

 

GBR26

 

(CT)15 (CA)13

60

F: TGGCCACACGAGATTATTCA

103-164

90

7

7.0

0.100

0.139

0.282*

JX661260

F: ATATCGGGCCHTTTCACA

 

GBR28

 

CA)26

60

F: ACAACACGCCTCAGTTCACA

195

89

1

-

-

-

-

JX661261

F: GCTGCCTTGAGTGAGTCTCC

 

GBR29

 

(GT)110

60

F: GGCGTGCTTGTGTATAGGAA

132-174

97

3

2.9

0.010

0.031

0.666*

JX661262

F: CCCAGCAGGGTTGCTTAG

 

GBR30

 

(CA)13

60

F: CGCACACTTATCCTGTCGTG

198-206

98

2

2.0

0.010

0.030

0.664*

JX661263

F: GACAGTGAGTCATGTGTGCATTT

 

Mean (all indiv.)2

     

84.9

3.6

3.6

0.107

0.125

0.142*

 

B) Wild individuals

GBR9

     

56

3

2.9

0.180

0.179

-0.90

 
 

GBR20

     

56

2

2

0.053

0.052

-0.019

 
 

GBR24

     

54

2

2

0.494

0.490

-0.127

 
 

GBR26

     

53

4

4

0.074

0.073

-0.017

 
 

GBR29

     

59

2

1.9

0.036

0.036

1.000*

 
 

GBR30

     

56

2

1.9

0.018

0.018

0.000

 
 

Mean (all wild indv.)

     

55,7

2.5

3.1

0.143

0.141

-0.052

 

C) Captive samples

GBR9

     

42.0

3

2.98

0.114

0.115

0.382

 
 

GBR11

     

41.0

2

1.99

0.048

0.048

1.000

 
 

GBR16

     

36.0

2

2.00

0.080

0.081

0.660

 
 

GBR20

     

41.0

5

4.62

0.117

0.119

0.592

 
 

GBR24

     

42.0

4

3.71

0.475

0.481

-0.040

 
 

GBR26

     

37.0

7

6.92

0.226

0.229

0.413

 
 

GBR29

     

42.0

2

1.86

0.023

0.024

0.000

 
 

GBR30

     

42.0

2

1.98

0.046

0.047

1.000*

 
 

Mean (all captive indv.)

     

40.4

3.4

3.26

0.141

0.143

0.511

 
  1. N= number of individuals assayed. N A = number of alleles per locus. N AR =allelic richness standardized to the smallest sample size using the rarefaction method of FSTAT 2.9.3 [73]. expected (H E ) and observed (H O ) heterozygosities. F IS = Wright’s statistics.
  2. 1Bold F IS values are significant probability estimates after q-value correction (*p<0.05).
  3. 2The mean values were calculated only with the polymorphic loci data.
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BMC Genomic Data

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