Fig. 4From: Quantitative high resolution melting: two methods to determine SNP allele frequencies from pooled samplesAnalysis of 384 loci in six individuals (Peaks method). Six adult corals were HRM-genotyped individually for 384 SNPs, then qHRM-genotyped as a pool in triplicate. The expected allele frequency was calculated by summing the genotypes for each individual (12 alleles possible), and the qHRM-estimated frequencies were calculated as described in the Materials and Methods section. The Pearson correlation between the expected and the qHRM-estimated values is 0.89. The slope of the linear regression is 0.94Back to article page