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Fig. 4 | BMC Genetics

Fig. 4

From: MECP2, a gene associated with Rett syndrome in humans, shows conserved coding regions, independent Alu insertions, and a novel transcript across primate evolution

Fig. 4

RT-PCR products corresponding to novel transcripts in Callimico goeldii. a RT-PCR products following amplification of a 525 bp contig resulted in two fragments of 403 and 279 bp. b Transcript composition (E1 = exon 1; E2 = exon 2). Arrows indicate the primer positions. The new alternative exon, homologous to part of human intron 2, is shown in black. c Schematic representation of the novel exon and the alternative splicing resulting in the two new transcripts. Start and stop codons indicate three likely open reading frames. IN1 = intron 1; IN2 = intron 2; IN3 = intron 3; E1 = exon 1; E2 = exon 2; E3 = exon 3

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