Fig. 7

daf-16 nuclear translocation does not enhance daf-2(rf) freezing survival. (a–d, i) daf-2 RNAi enhanced daf-16::GFP nuclear expression compared with TJ356 [41] animals (proportion daf-16::GFP nuclear translocation: class 1 = 0 %, class 2 > 0 %–50 %, class 3 > 50 %–100 %). (e–h, j) No difference in daf-16::GFP nuclear expression was found between TJ356 and daf-2(RNAi) animals with freeze–thaw stress. Every assay was repeated at least three times in the same conditions. Statistical significance was assessed by the Mann–Whitney test. **p < 0.01, n.s. - not significant