From: Mutational and clinical analysis of the ENG gene in patients with pulmonary arterial hypertension
EXON | PRIMERS | SIZE | Tª | |
---|---|---|---|---|
Forward 5’ → 3’ | Reverse 5’ → 3’ | |||
1 | ACTGGACACAGGATAAGGCCCAGC | AATACTTGGGGCCTGGTCCGTG | 180 bp | 62 °C |
2 | CACCTTATTCTCACCTGGCCTCTT | CTGCCTTGGAGCTTCCTCTGAG | 249 bp | 61 °C |
3 | GGGTGGCACAACCTATACAAAT | CAGAGATGGACAGTAGGGACCT | 269 bp | 60 °C |
4 | CTACATGGGATAGAGAGGGCAC | TTCCTCCTGAGCAGTATCATGAG | 277 bp | 55 °C |
5 | TGAGGGAAGGGACTGAGGTGC | GTGGGGACTAGTGTCAGGGGC | 238 bp | 63 °C |
6 | GGCCTGTCCGCTTCAGTGTT | GTTTTGTGTCCCGGGAGCTG | 203 bp | 58 °C |
7 | CCCCCTGTTCTGCCTCTCTC | CTGATCCAAGGGAGGGGAAG | 256 bp | 63 °C |
8 | ACACATATCACACAGTGACCAGC | CTAGGGGAGGAACCAGATGTC | 253 bp | 55 °C |
9 | CTCCTGATGGTGCCCCTCTCTTC | TTGTCTTGTGTTCTGAGCCCCTG | 296 bp | 60 °C |
10 | CTGCAGGGGCTCAGAACACA | GGCCAGGTGGGTTAGCACG | 212 bp | 61 °C |
11 | ATTGACCAAGTCTCCCTCCC | GAAAGGCGGAGAGGAAGTTC | 211 bp | 61 °C |
12 | GGTGGGGTGAAGAGCAGCTG | GACCTGGAAGCTCCCACTTGAA | 359 bp | 58 °C |
13 | GAGTAAACCTGGAAGCCGC | GCCACTAGAACAAACCCGAG | 154 bp | 55 °C |
14 A | CCAGCACAACAGGGTAGGGGAT | CTCAGAGGCTTCACTGGGCTCC | 255 bp | 61 °C |
14 B | AGGACCCTGACCTCCGCC | CTCTCCTGCTGGGCGAGC | 198 bp | 63 °C |