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Table 1 Mutations in CAN1 are mostly large-scale changes, while mutations in URA3 are mostly small-scale changes

From: Growth conditions that increase or decrease lifespan in Saccharomyces cerevisiae lead to corresponding decreases or increases in rates of interstitial deletions and non-reciprocal translocations

Sample CAN1 mutations URA3 mutations
Untreated + MMSa Untreated + MMSa
N Freq His+ N Freq His+ N Freq point Mutationsb N Freq point mutationsb
YPD 217 0.24 110 0.46 99 0.95 43 0.98
SC + 2 % Glucose 137 0.24 80 0.41 57 1.0 13 0.92
30 °C 102 0.28 66 0.47 27 0.96 16 1.0
10 % Glucose 111 0.21 83 0.46 33 0.97 50 0.94
0.3 M NaCl 135 0.26 81 0.41 46 1.0 39 0.97
3 % Acetate 98 0.18 78 0.33 37 0.81* 19 1.0
0.2 % Glucose 106 0.27 82 0.38 38 0.87 50 0.96
2 % Ethanol 108 0.39** 96 0.34 46 0.80* 40 0.95
YPD rad52∆ 59 0.88*** NTc NTc 25 0.96 NTc NTc
  1. aChronic exposure to 200 μM (0.0022 %) MMS
  2. bScored as point mutation if yielded expected size URA3 PCR product
  3. cNot tested
  4. Asterisks indicate levels of significance: * = p < 0.05, ** = p < 0.01, *** = p < 0.001