Fig. 6

DmManf interacts with genes involved in the ubiquinone synthesis pathway. a Quantitative RT-PCR analysis showed increased DmManf mRNA level when COQ7 was ubiquitously knocked down with tub-GAL4. UAS-lacZ was used as a wild type control. b A schematic presentation of ubiquinone synthesis pathway according to Kyoto Encyclopedia of Genes and Genomes (http://www.genome.jp/kegg/, pathway ID 00130). Genes marked with yellow were found to genetically interact with DmManf. c UAS-RNAi lines of genes involved in ubiquinone synthesis pathway were crossed to MS1096-GAL4 and 69B-GAL4 drivers in wild type, heterozygous DmManf ^Δ96 mutant or DmManf overexpression background. See Fig. 4 legend for interpretation and Additional file 2 for schematic presentation of the crosses. Het, heterozygous DmManf ^Δ96 mutant; MAA, microarray analysis; ND, not determined; OE, overexpression