Table 1 Primers used for long-range PCR, sequencing and genotyping of ovine BMP15 and GDF9 genes
Gene | Primer sequence (5′→3′) | Location | Method |
|---|---|---|---|
BMP15 | TTCCTTGCCCTATCCTTTGTG | exon 1 | long-range PCR/seq. |
BMP15 | GAGGCCTTGCTACACTAGCC | exon 1 | genotypingFecX + |
BMP15 | TGAGAGGCCTTGGCTACACA | exon 1 | genotypingFecX Bar |
BMP15 | ACTTTTCTTCCCCATTTTTCTGC | exon 1 | sequencing |
BMP15 | CGCTTTGCTCTTGTTCCCTC | exon 2 | sequencing |
BMP15 | GGCACTTCATCATTGGACACT | exon 2 | sequencing |
BMP15 | GGCAATCATACCCTCATACTCC | exon 2 | sequencing |
BMP15 | TCTTCACCCCAAACCGTCTA | exon 2 | long-range PCR/seq. |
GDF9 | GAAGACTGGTATGGGGAAATG | exon 1 | long-range PCR/seq. |
GDF9 | CCAATCTGCTCCTACACACCT | exon 1 | sequencing |
GDF9 | TGGCATTACTGTTGGATTGTTTT | exon 2 | sequencing |
GDF9 | TGAACGACACAAGTGCTCAGG | exon 2 | sequencing |
GDF9 | GATAGCCCTCTCTTCTGGTCA | exon 2 | sequencing |
GDF9 | GCTCCTCCTTACACAACACACAG | exon 2 | sequencing |
GDF9 | GTGTTCTTACCTGGCAAACC | exon 2 | long-range PCR/seq. |