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Fig. 4 | BMC Genetics

Fig. 4

From: High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in Bangladesh

Fig. 4

HRM curve patterns for unknown samples using 1st set of primers (P1R1) covering exon-1 and a portion of intron-1. The normalized and temperature shifted difference curve could separate various combinations of mutations including homozygous (#), heterozygous (*) or compound heterozygous (*/*) states from wild type alleles and from each other

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