Fig. 5From: High resolution melting curve analysis targeting the HBB gene mutational hot-spot offers a reliable screening approach for all common as well as most of the rare beta-globin gene mutations in BangladeshThe HRM curve analysis for unknown samples using the 2nd set of primers (P2R2) covering a portion of intron-1 and exon-2. The normalized and temperature shifted difference curve could separate various mutations from the wild type alleles and from each otherBack to article page