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Table 1 Primers sequences used to amplify the Bm86 cDNA by rt-PCR

From: Genetic diversity of Bm86 sequences in Rhipicephalus (Boophilus) microplus ticks from Mexico: analysis of haplotype distribution patterns

Primer aSequence bTm/sec cSize (bp)
Bm86_ F1 5’-GTGGTTCGACGCAGTGAGAT-3′ 60°/30 680
R1 5’-CCATGCTTGCAGACAAACCC-3´
F2 5’-CTGCAAAGACCTCTGCGAGA-3´ 55°/30 686
R2 5’-TGTCCTGCGTGCAGTTAAGT-3´
F3 5’-CGGGCCCAAATGTCAACATC-3´ 55°/30 680
R3 5’-AACGCACTCCAGCTTCTTGT-3´
F4 5’-AAAACGAGCAGTCGGAGTGT-3´ 57°/30 542
R4 5’-GGTGTTCGATGTAAGCGTGA-3´
  1. aPrimer sequence, directionality is indicated as forward (F) and reverse (R)
  2. bMelting temperature in Celsius degrees/incubation time in seconds
  3. cAmplicon size in base pairs are listed for each pair of primers