Polymorphism of MHC class IIB in an acheilognathid species, Rhodeus sinensis shaped by historical selection and recombination

Table 1 PCR primers used for the amplification of DAB sequences in Rhodeus sinensis. Data comprise primer name, direction (D), sequence, priming position (Position) and reference (Ref)

Name	D	Sequence (5′ - 3′)	Position	Ref
FishIIBEx2-1F	F	CTGATGCTGTCTGCTTTCACTGGA	SP	1
FIBE-F1	F	CTGATGCTGTCTGCTTTCAC	SP	P
ROSI-R1	R	CGGGATCCCAGTCTACGATG	TM	P
ROSI-R2	R	GATCCCAGTCTACGATGATG	TM	P
ROSI-R3	R	GAGTGTATTCTAGGTGAGAGTGG	exon3 3′ end	P
ROSI-R4	R	CAGGTGCGAGTGAATCTGGT	exon3 middle	P
ROSI-R5	R	CTGAGCTTGACCTTTGGTGC	exon3 5′ end	P
Rooc-Fw	F	CCCATAGTTGACATGATGTCATCTG	intron1	2
Rooc-Rev	R	CATGTGTGACAGGAGGATCAG	intron2	2
DAB1-Rev374	R	CAAGAGTTTCCCGTGTGACAG	intron2	2
DAB1-Fw18	F	AGTTGACATGATGCCATCTGA	intron1	2
DAB-Ex1-Fw	F	CATCCATACTGATGCTGTCTGC	SP	2
Rhod-GSP2	R	CCAGTCTCCGTTAGGCATCTCC	exon3	2
SP-F1	F	CTGATGCTGTCKRCWTTYACYGGA	SP	P
TM-R1	R	TGGTACCAGGATCCTCCC	TM	P
TM-R2	R	TGGKACCAGGATYCTCCC	TM	P
MHC2-TMr	R	TCAGTTTGGTACCAGGATCCTCC	TM	P
Exon-3R	R	TGAGCTTGACCTCTGGTGCCAC	exon3 5′ end	P
THMHCI-3R	R	GCCAGCGTGATCCACCATAC	TM	P

(Ref) 1: Ottová et al., 2007 [41]; 2: Agbali et al., 2010 [33]; P: present study

ISSN: 2730-6844