Table 3 Nucleotide sequences of primers used for promoter amplification and BS-PCR in this study
Primer name | Nucleotide sequence (5′ to 3′) |
|---|---|
For promoter amplification | |
Sox9a-P1 | GACTCAGAGGAAAGCCAAGC |
Sox9a-C1 | TCTTGAAGTCTGCGAGGCGG |
Amh-P1 | GTTGGAAACCTTGGCTGTCT |
Amh-C1 | GTCGCCACAATCAGCAACAG |
Cyp19ala-P1 | TTAGCGATGAAAGTGGGCGT |
Cyp19ala-C1 | TGTCCGATGGTGTCTGATGG |
For BS-PCR | |
Sox9a-F3 | TATAAAAATGAATTTTGAAAGATGT |
Sox9a-R3 | ATCTACAAATCATATTTCAATTTATC |
Amh-F3 | TAGTATGATGTATAAAGATGAATGT |
Amh-R3 | TACTCCTAGCCCTGATCTCTGGA |
Cyp19ala-F3 | ACAACACCTCAAATAAACCCTAC |
Cyp19ala-R3 | CAAAAAAACCTCACTAAAATACACC |