Fig. 3
From: Further identification of a 140bp sequence from amid intron 9 of human FMR1 gene as a new exon
The new spliced transcript containing the 140 bp sequence is translated into a truncated FMRP protein with altered subcellular localization. a Western blots showing the expression of the wild type FMRP or the truncated FMRP in HEK293T cells. 1: untransfected HEK293T cells; 2: HEK293T cells transfected by pEGFP-N2-tFMR1; 3: HEK293T cells transfected by pEGFP-N2-fFMR1. b Immunofluorescence showing the localization of the wild type FMRP or the truncated FMRP in HEK293T cells. HEK293T: untransfected HEK293T cells; pEGFP-N2: HEK293T cells transfected by the empty vector; pEGFP-N2-tFMR1: HEK293T cells transfected by pEGFP-N2-tFMR1; pEGFP-N2-fFMR1: HEK293T cells transfected by pEGFP-N2-fFMR1; DAPI: the solution suitable for nuclear staining; EGFP: vectors encoding the GFP-tagged protein; Alexa Flour 594: a FMRP antibody fluorescently labeled red. c Quantitative representation of EGFP positive cells. d Quantitative representation of FMRP positive cells