Table 5 Primers used for real-time quantitative PCR
Target genes | Primer Sequences(5′~ 3′) | Tm (°C) | GenBank Accession No. |
|---|---|---|---|
SCD1 | F: TCCTGTTGTTGTGCTTCATCC R: GGCATAACGGAATAAGGTGGC | 59 | AY241933 |
FAS | F: GCAAAGTGGTCATTCAGGTACG R: CCCAGTGATGATGTAGCTCTTG | 60 | KM980092.1 |
PPARG | F: CAGTGTCTGCAAGGACCTCA R: GTAAAAGGCATGGGAGTGAT | 59 | HG270143.1 |
SREBP1 | F: CTGACGACCGTGAAAACAGA R: AGACGGCAGATTTATTCAACTT | 58 | KU517672.1 |
GPAT | F: GCAGGTTTATCCAGTATGGCATT R: GGACTGATATCTTCCTGATCATCTTG | 59 | AY515690.1 |
AGPAT6 | F: AAGCAAGTTGCCCATCCTCA R: AAACTGTGGCTCCAATTTCGA | 59 | DY208485 |
ACACA | F: TCCTGCTGCTATCGCTACTCCA R: CGCACTCACATAACCAACCAT | 61 | DQ773054.1 |
GAPDH | F: TGGAAAGGCCATCACCATCT R:CCCACTTGATGTTGGCAG | 60 | NM001034034.1 |