Table 2 Associations of the SNPs in KLF6 gene with milk production traits in two lactations in Chinese Holstein (LSM ± SE).

g.44,595,327 A > G

AA(165)

6928.35 ± 255.38

263.82 ± 10.364^a

3.799 ± 0.102^a

219.8 ± 5.025^a

3.165 ± 0.037^a

AG(540)

6915.51 ± 254.56

259.4 ± 10.34^a

3.736 ± 0.102^b

214.69 ± 5.027^b

3.125 ± 0.037^b

GG(412)

6808.59 ± 254.37

254.3 ± 10.333^b

3.722 ± 0.102^b

212.6 ± 5.025^b

3.131 ± 0.037^b

P value

0.0509

0.0009**

0.0155*

< 0.0001**

0.0001**

AA(107)

9686.93 ± 142.82^a

372.34 ± 5.951^a

3.833 ± 0.058^a

303.62 ± 4.339^a

3.147 ± 0.02

AG(307)

9442.31 ± 129.56^b

352.4 ± 5.462^b

3.758 ± 0.053^b

293.01 ± 3.981^b

3.129 ± 0.018

GG(200)

9460.75 ± 132.74^b

349.51 ± 5.568^b

3.696 ± 0.054^c

296.97 ± 4.059^b

3.151 ± 0.018

P value

0.0052**

< 0.0001**

0.0001**

< 0.0001**

0.04*

g.44595580G > A

AA(412)

6629.68 ± 250.39

249.98 ± 10.157^b

3.775 ± 0.1^b

203.9 ± 4.905^b

3.119 ± 0.036^a

AG(542)

6722.68 ± 250.81

255.81 ± 10.176^a

3.806 ± 0.1

204.96 ± 4.918^b

3.117 ± 0.036^a

GG(163)

6754.22 ± 252.28

260.12 ± 10.23^a

3.861 ± 0.101^a

209.69 ± 4.937^a

3.155 ± 0.036^b

P value

0.074

0.0002**

0.0055**

< 0.0001**

0.0002**

AA(200)

9566.8 ± 133.86^b

344.42 ± 5.611^b

3.616 ± 0.054^b

304.7 ± 4.09^b

3.177 ± 0.019

AG(307)

9614.5 ± 130.15^b

349.3 ± 5.488^b

3.67 ± 0.053^b

303.73 ± 4^b

3.162 ± 0.018

GG(107)

9831.6 ± 141.34^a

368.39 ± 5.895^a

3.75 ± 0.057^a

313.34 ± 4.298^a

3.181 ± 0.02

P value

0.0042**

< 0.0001**

0.0002**

< 0.0001**

0.1567

 g.44595695G > A

AA(411)

6830.13 ± 249.78

247.71 ± 10.116^b

3.662 ± 0.1

208.46 ± 4.868^b

3.116 ± 0.036^b

AG(541)

6906.41 ± 250.41

254.04 ± 10.14^a

3.702 ± 0.1

210.18 ± 4.877

3.117 ± 0.036^b

GG(166)

6898.9 ± 251.68

253.42 ± 10.194

3.722 ± 0.101

212.35 ± 4.911^a

3.155 ± 0.036^a

P value

0.252

0.0029**

0.0335*

0.0075**

< 0.0001**

AA(199)

9471.29 ± 132.89^b

349.54 ± 5.575^b

3.692 ± 0.054^b

297.44 ± 4.064^b

3.154 ± 0.018^a

AG(307)

9432.68 ± 129.56^b

351.85 ± 5.461^b

3.757 ± 0.053^b

292.56 ± 3.981^c

3.127 ± 0.018^b

GG(108)

9678.43 ± 142.38^a

372.25 ± 5.933^a

3.835 ± 0.058^a

303.48 ± 4.326^a

3.149 ± 0.02

P value

0.0059**

< 0.0001**

< 0.0001**

< 0.0001**

0.0096**

g.44,595,808 C > T

CC(615)

6951.08 ± 250

258.45 ± 10.158

3.723 ± 0.1

214.74 ± 4.94^b

3.117 ± 0.036

CT(434)

6912.47 ± 251.39

257.52 ± 10.217

3.73 ± 0.101

213.44 ± 4.975^b

3.109 ± 0.036^b

TT(68)

7008.87 ± 261.67

260.58 ± 10.617

3.709 ± 0.105

222.01 ± 5.146^a

3.144 ± 0.038^a

P value

0.4903

0.6825

0.8303

< 0.0001**

0.0341*

CC(357)

9501.42 ± 129.08^a

353.3 ± 5.443

3.725 ± 0.052

296.64 ± 3.967^a

3.14 ± 0.018

CT(223)

9466.48 ± 132.05^a

354.22 ± 5.547

3.766 ± 0.054

294.86 ± 4.044^a

3.137 ± 0.018

TT(34)

9127.73 ± 171.65^b

345.14 ± 7.009

3.804 ± 0.069

284.4 ± 5.112^b

3.12 ± 0.024

P value

0.0096**

0.1787

0.0854

0.0021**

0.5218

 g.44,596,221 A > C

AA(614)

6643.76 ± 252.53

263.94 ± 10.247

3.943 ± 0.101

203.2 ± 4.991^a

3.123 ± 0.036

AC(432)

6547.53 ± 254.24

260.41 ± 10.324

3.948 ± 0.102

200.21 ± 5.044^b

3.12 ± 0.037

CC(67)

6702.38 ± 264.48

268.41 ± 10.725

3.963 ± 0.106

206.73 ± 5.217^a

3.14 ± 0.038

P value

0.056

0.0383*

0.8526

< 0.0001**

0.3585

AA(355)

9551.93 ± 130.72^a

352.35 ± 5.517

3.677 ± 0.053

308.12 ± 4.021^a

3.22 ± 0.018

AC(222)

9464.89 ± 134.17^a

351.19 ± 5.642

3.717 ± 0.054

303.82 ± 4.112^b

3.209 ± 0.019

CC(32)

9150.13 ± 177.15^b

344.95 ± 7.229

3.774 ± 0.071

295.13 ± 5.272^b

3.2 ± 0.025

P value

0.0041**

0.312

0.0557

0.0002**

0.2886

 g.44,596,271 C > T

CC(526)

6788.7 ± 249.29^b

250.62 ± 10.096^a

3.727 ± 0.1^a

208.53 ± 4.86^b

3.139 ± 0.036^a

CT(487)

6983.21 ± 249.35^a

253.75 ± 10.101^a

3.665 ± 0.1^b

213.59 ± 4.864^a

3.123 ± 0.036^b

TT(104)

6706.52 ± 257.3^b

242.32 ± 10.412^b

3.664 ± 0.103

202.46 ± 5.001^c

3.104 ± 0.037^b

P value

< 0.0001**

0.0008**

0.0019**

< 0.0001**

0.0022**

CC(304)

9397.7 ± 130.62^b

364.89 ± 5.5^a

3.917 ± 0.053^a

299.72 ± 4.009^b

3.194 ± 0.018

CT(258)

9372.63 ± 131.9^b

349.18 ± 5.549^b

3.769 ± 0.054^b

298.57 ± 4.045^b

3.194 ± 0.018

TT(51)

9722.82 ± 157.38^a

354.6 ± 6.482^b

3.64 ± 0.063^c

314.57 ± 4.727^a

3.227 ± 0.022

P value

0.0032**

< 0.0001**

< 0.0001**

< 0.0001**

0.0841

 g.44,596,874 A > G

AA(169)

6621.56 ± 260.53

251.31 ± 10.558

3.796 ± 0.104

208.09 ± 5.123^a

3.157 ± 0.038^a

AG(502)

6742.08 ± 259.51^a

256.1 ± 10.52^a

3.797 ± 0.104

207.99 ± 5.112^a

3.125 ± 0.038^b

GG(410)

6621.1 ± 259.41^b

248.33 ± 10.521^b

3.754 ± 0.104

204.89 ± 5.118^b

3.125 ± 0.037^b

P value

0.0227*

0.0003**

0.0618

0.0014**

0.0016**

AA(105)

9626.84 ± 144.15^a

349.38 ± 6.022^a

3.67 ± 0.058^a

309.84 ± 4.39^a

3.216 ± 0.02

AG(287)

9363.44 ± 134.21^b

330.33 ± 5.656^b

3.603 ± 0.054^a

298.9 ± 4.123^b

3.202 ± 0.018^b

GG(198)

9424.69 ± 138.2^b

327.25 ± 5.804^b

3.524 ± 0.056^b

304.46 ± 4.231^a

3.227 ± 0.019^a

P value

0.0039**

< 0.0001**

< 0.0001**

< 0.0001**

0.0339*

g.44,600,440 C > T

CC(653)

6990.22 ± 250.02^a

256.28 ± 10.152^a

3.686 ± 0.1^a

216.18 ± 4.927^a

3.116 ± 0.036

CT(407)

7065.68 ± 250.65^a

256.04 ± 10.18^a

3.64 ± 0.1^b

217.15 ± 4.949^a

3.105 ± 0.036

TT(56)

6575.48 ± 265.06^b

245.03 ± 10.757^b

3.72 ± 0.106

202.51 ± 5.23^b

3.112 ± 0.038

P value

< 0.0001**

0.0189*

0.017*

< 0.0001**

0.2944

CC(368)

9467.17 ± 129.76^a

364.43 ± 5.474^a

3.888 ± 0.053^a

300.43 ± 3.99^b

3.182 ± 0.018^c

CT(215)

9266.39 ± 133.32^b

347.15 ± 5.594^b

3.775 ± 0.054^b

297.32 ± 4.078^b

3.211 ± 0.018^b

TT(30)

9711.85 ± 174.73^a

353.83 ± 7.13

3.656 ± 0.07^b

317.67 ± 5.201^a

3.266 ± 0.025^a

P value

0.0002**

< 0.0001**

< 0.0001**

< 0.0001**

< 0.0001**

g.44601035G > A

AA(58)

6457.58 ± 264.57^c

250.54 ± 10.739^b

3.82 ± 0.106

200.21 ± 5.21^c

3.136 ± 0.038

AG(394)

6921.12 ± 251.62^a

262.73 ± 10.226^a

3.771 ± 0.101

211.76 ± 4.976^a

3.113 ± 0.036

GG(658)

6803.97 ± 250.4^b

259.4 ± 10.176

3.795 ± 0.1

208.91 ± 4.949^b

3.123 ± 0.036

P value

< 0.0001**

0.0057**

0.2895

< 0.0001**

0.174

AA(32)

10,135 ± 173.73^a

359.67 ± 7.09

3.554 ± 0.07^b

327.74 ± 5.172^a

3.242 ± 0.025^a

AG(207)

9654.12 ± 133.79^c

350.06 ± 5.63^b

3.653 ± 0.054^b

307.73 ± 4.103^b

3.198 ± 0.018^a

GG(372)

9806.93 ± 131.91^b

365.22 ± 5.56^a

3.766 ± 0.054^a

309.28 ± 4.053^b

3.172 ± 0.018^b

P value

0.0004**

< 0.0001**

< 0.0001**

< 0.0001**

< 0.0001**

g.44,601,887 C > T

CC(468)

6862.52 ± 249.21^a

252.95 ± 10.093^a

3.722 ± 0.1^a

210.89 ± 4.859^a

3.141 ± 0.036^a

CT(521)

6954.1 ± 249.61^a

252.42 ± 10.109^a

3.662 ± 0.1^b

212.4 ± 4.866^b

3.123 ± 0.036^b

TT(138)

6655.56 ± 254.64^b

240.09 ± 10.309^b

3.66 ± 0.102

200.42 ± 4.958^b

3.102 ± 0.037^b

P value

< 0.0001**

< 0.0001**

0.0028**

< 0.0001**

0.0003**

CC(272)

9466.9 ± 132.53

366.55 ± 5.573^a

3.904 ± 0.054^a

302.28 ± 4.062^b

3.197 ± 0.018

CT(271)

9346.91 ± 130.18^a

352.85 ± 5.484^b

3.816 ± 0.053^b

297.69 ± 3.997^c

3.193 ± 0.018^b

TT(70)

9599.7 ± 150.37^b

348.49 ± 6.221^b

3.63 ± 0.061^c

310.03 ± 4.536^a

3.226 ± 0.021^a

P value

0.0113*

< 0.0001**

< 0.0001**

< 0.0001**

0.0441*

g.44602809G > T

GG(856)

6866.89 ± 252.49^a

257.52 ± 10.247

3.741 ± 0.101

215.45 ± 4.972^a

3.135 ± 0.036^b

GT(250)

6723 ± 255.19^b

252.72 ± 10.362

3.76 ± 0.102

211.65 ± 5.035^b

3.155 ± 0.037^a

TT(10)

6949.16 ± 328.85

258.12 ± 13.253

3.758 ± 0.131

220.38 ± 6.286

3.187 ± 0.048

P value

0.0206*

0.0735

0.6532

0.0003**

0.0138*

GG(464)

9540.27 ± 128.29^a

359.12 ± 5.416^a

3.78 ± 0.052

298.36 ± 3.947^a

3.15 ± 0.018^a

GT(140)

9474.07 ± 136.04^a

356.36 ± 5.688^a

3.789 ± 0.055

293.69 ± 4.146^b

3.12 ± 0.019^b

TT(9)

8906.48 ± 259.78^b

332.56 ± 10.358^b

3.773 ± 0.104

271.51 ± 7.559^c

3.074 ± 0.038

P value

0.0172*

0.0096**

0.9404

< 0.0001**

0.0016**

g.44603687G > A

AA(419)

6826.6 ± 249.82

247.54 ± 10.117^b

3.66 ± 0.1^b

208.66 ± 4.867^b

3.118 ± 0.036^b

AG(536)

6899.47 ± 250.36

253.51 ± 10.138^a

3.7 ± 0.1

209.67 ± 4.876

3.114 ± 0.036^b

GG(163)

6906.15 ± 251.7

253.89 ± 10.195

3.725 ± 0.101^a

212.59 ± 4.912^a

3.155 ± 0.036^a

P value

0.249

0.0035**

0.0226*

0.0084**

< 0.0001**

AA(203)

9453.37 ± 132.73^b

351.16 ± 5.568^b

3.715 ± 0.054^a

296.45 ± 4.059^b

3.15 ± 0.018

AG(306)

9450.28 ± 129.55^b

351.05 ± 5.461^b

3.742 ± 0.053^a

293.46 ± 3.981^b

3.13 ± 0.018

GG(105)

9660.5 ± 142.9^a

372.47 ± 5.954^a

3.844 ± 0.058^b

302.66 ± 4.34^a

3.147 ± 0.02

P value

0.0183*

< 0.0001**

0.0003**

0.0002**

0.073