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Table 1 The distribution of curve magnitude among curveback mapping offspring

From: A major QTL controls susceptibility to spinal curvature in the curveback guppy

  Qualitative value for curve magnitude Total offspring Total curved Percent curved
  0 1 2 3 4    
Backcross 67 32 52% 23 37% 5 8% 2 3% 129 62 48%
F2 - Cross 1 224 40 64% 16 26% 6 10% 0 n/a 286 62 22%
F2 - Cross 2 200 11 19% 35 59% 10 17% 3 5% 259 59 29%
  1. Backcross offspring are from Cross 1. Fish were scored for curve magnitude using a qualitative scale [7]: 0 = non-curved to 4 = extreme curve. Mapping offspring are euthanized at a minimum of 3 months past birth (sexual maturity is at about 1 month past birth), photographed, and frozen in buffered (EDTA) ethanol. The same person scored each individual alive, and then scores were confirmed in photos. Among each phenotypic class (score), the proportion of fish among curved individuals is shown. All fish were photographed on a light table with a digital camera under 3X magnification.