- Research article
- Open Access
Characterisation of the genomic architecture of human chromosome 17q and evaluation of different methods for haplotype block definition
© Zeggini et al; licensee BioMed Central Ltd. 2005
Received: 17 November 2004
Accepted: 25 April 2005
Published: 25 April 2005
The selection of markers in association studies can be informed through the use of haplotype blocks. Recent reports have determined the genomic architecture of chromosomal segments through different haplotype block definitions based on linkage disequilibrium (LD) measures or haplotype diversity criteria. The relative applicability of distinct block definitions to association studies, however, remains unclear. We compared different block definitions in 6.1 Mb of chromosome 17q in 189 unrelated healthy individuals. Using 137 single nucleotide polymorphisms (SNPs), at a median spacing of 15.5 kb, we constructed haplotype block maps using published methods and additional methods we have developed. Haplotype tagging SNPs (htSNPs) were identified for each map.
Blocks were found to be shorter and coverage of the region limited with methods based on LD measures, compared to the method based on haplotype diversity. Although the distribution of blocks was highly variable, the number of SNPs that needed to be typed in order to capture the maximum number of haplotypes was consistent.
For the marker spacing used in this study, choice of block definition is not important when used as an initial screen of the region to identify htSNPs. However, choice of block definition has consequences for the downstream interpretation of association study results.
Recent advances in high-throughput genotyping technologies have realised the possibility of performing large-scale, high-resolution genetic studies in human complex diseases. Single nucleotide polymorphisms (SNPs) have become the markers of choice due to their frequent occurrence, simple mutational dynamics and the fact that they lend themselves to automated allele calling [1, 2]. The number of SNPs with allele frequencies higher than 10% has been estimated to exceed 5,000,000 . Exhaustive genome-wide association studies, thereby, reach prohibitive costs and require ultra-high throughput technologies. Comprehensive SNP screening of regions or genes of interest is both inefficient and unnecessary, as information redundancy can arise from linkage disequilibrium (LD). A common strategy in complex disease association studies is the selection and genotyping of a subset of SNPs, assumed to be in LD with the untested polymorphisms. In the past, association study designs have not selected markers on a strong scientific basis, due to restricted comprehension of LD patterns. Gaining a better understanding of the LD blueprint of the human genome can now facilitate disease gene mapping, as sets of non-redundant SNPs can be employed to design cost-effective strategies [4–6]. SNP maps utilised by current genetic studies concentrating on chromosomal regions cover a wide spectrum of marker spacing intervals, ranging from ~50 kb [7, 8] to 15–20 kb [9, 10] to high resolution maps of approximately 1 SNP every kb [11, 12].
Patterns of LD across the genome have been shown to be variable and found to be a property of individual chromosomal regions rather than a simple monotonic function of physical distance between markers [6, 9, 13–15]. Regions of low haplotype diversity interspersed by regions of low LD (termed haplotype blocks) have been empirically identified and proposed to constitute a ubiquitous feature of the genome [16–18]. Their presence has triggered funding of the Haplotype Map project, leading to the generation of a genome-wide index of common blocks. Characterisation of haplotype blocks can provide association studies with a shortcut to screening chromosomal regions for the presence of disease variants through the identification of haplotype-tagging SNPs (htSNPs) and can additionally aid in interpreting the results of initial scans through knowledge of the underlying genetic architecture [5, 19–22].
The potential benefits of utilising haplotype blocks may, however, be challenged by concerns regarding their consistency and, hence, applicability to different populations, the information loss incurred by examining common variation and the arbitrary choice of block definition . Different studies investigating the structure of haplotype blocks have used distinct definitions based on various subjective criteria. Block definition methods can be broadly classified into three categories: those based on measures of LD [16, 24], those based on haplotype diversity [11, 25, 26] and those combining both approaches [9, 27]. Methods based on LD measures generally define blocks as regions in which all pairwise LD coefficients exceed a subjective threshold. Methods based on haplotype diversity generally define blocks as regions in which a small, arbitrary number of haplotypes accounts for a predefined percentage of the observed variation. The consensus finding is that denser marker maps, larger sample sizes and use of common variants lead to shorter blocks [23, 24]. However, the extent of difference in block structure, to which distinct haplotype block definitions and thresholds may result in, remains unclear. The size and number of generated blocks could have an impact on the downstream analysis of association studies and could, therefore, influence the design of fine mapping strategies to identify disease-causing variants.
In the present study, we address these issues by applying different haplotype block definition criteria to 137 SNPs, in order to describe the genetic architecture of a 6.1 Mb region of 17q in a set of 189 unrelated healthy individuals. Employing methods based on both LD measures and haplotype diversity, we evaluate their relative merits and limitations, given our median marker spacing of 15.5 kb. Comparing the generated underlying block structures, we assess the usefulness and applicability of distinct methods in genetic association studies of complex human diseases.
DNA from a cohort of 189 healthy, unrelated, UK individuals of Euroepan ancestry was studied. Individuals were recruited from general practice or were blood donors. The collection was approved by the regional ethics committee.
Markers and genotyping
One hundred and thirty seven SNPs dispersed over 6.1 Mb of the human chromosomal 17q region were examined. We are currently investigating these markers as part of a fine mapping study for the identification of rheumatoid arthritis susceptibility genes. SNPs were selected from the SNP Consortium database  to span the region in equally spaced intervals. The SNP map of successfully genotyped markers was constructed based on the November 2002 Freeze of the Human Genome Sequencing Project, available through the UCSC Genome Browser .
Methodological details are available upon request from the authors and SNP IDs can be found in Additional file 1 [see Additional file 1]. Briefly, SNPs were genotyped using either the primer extension SNaPshot™ method (Applied Biosystems, CA, USA) through use of an ABI Prism 3100 DNA Analyzer and GeneScan® analysis software (Applied Biosystems, CA, USA), or the allelic discrimination 5' nuclease assay (TaqMan®, Applied Biosystems, CA, USA) through use of an ABI Prism 7700 platform (Applied Biosystems, CA, USA). All SNP genotype calls were independently checked by two individuals.
Departure from Hardy Weinberg equilibrium was initially assessed for each SNP. None of the SNPs were found to deviate from Hardy-Weinberg equilibrium significantly. Haplotypes were then inferred using the expectation-maximisation (EM) algorithm, either through the HelixTree™ (Golden Helix, Inc, Montana, USA) or the snphap (David Clayton, Cambridge, UK) software packages. Convergence of the algorithm was checked by repeating the haplotype estimation process 3 times, ensuring that identical results were generated.
Using SNP genotypes, the pairwise LD measure of D' was calculated. As values of D' can be overestimated with rare allele frequencies , the LD correlation coefficient r2 was additionally calculated for all pairs of SNPs. Observed D' and r2 values were sorted according to distance between the corresponding marker pairs. Running average D' and r2 values for sliding windows of 2 consecutive observations were estimated and plotted.
Haplotype block definitions
Haplotype block definitions were applied to the total set of 137 SNPs, as well as to the set of SNPs with allele frequencies exceeding 0.2 separately, in order to assess the effects of variant frequency on block structure.
Definition 1: The block definition method based on the D' measure of LD, employed by Gabriel et al. 2002 , was applied to the SNP genotype data through the HaploView software package (MJ Daly and JC Barrett, Whitehead Institute, MA, USA). Briefly, a block was defined as a region in which less than 5% of SNP pairs had a D' upper confidence bound less than 0.9. In addition, blocks consisting of 2 SNPs could span up to 20 kb and blocks of 3 or 4 SNPs could span up to 30 kb. Blocks were not allowed to overlap.
Definition 2: a; A simplified block definition method, also based on LD measures, was used. A haplotype block was defined as a region in which over 95% of all pairwise r2 LD correlation values exceeded 0.4. The same block length constraints as in Definition 1 were imposed, but a less rigid threshold was employed for stringency evaluation purposes. Blocks were allowed to overlap.b; For the subset of common SNPs, an additional method, based on D' values that tend to be overestimated for rare allele frequencies, was also employed. A haplotype block was defined as a region in which over 95% of all pairwise D' values exceeded 0.4. Blocks were allowed to overlap.
Definition 3: The block definition method proposed by Wang et al. 2002  was applied to the dataset. Briefly, a block was defined as a region in which, for all possible pairs of markers, less than four gametes were observed (D' = 1). Blocks were allowed to overlap.
Definition 4: A block definition method based on haplotype diversity was developed. For a set of n SNPs, the maximum number of haplotypes observed in the absence of recurrent mutation and / or recombination is n+1. Therefore, a haplotype block was defined as a region consisting of n SNPs, in which n+1 haplotypes could account for at least 95% of the observed variation. Taking each SNP as a seed, blocks were expanded or contracted to find the optimal window. Haplotype blocks were allowed to overlap.
Definition 5: A further block definition method based on LD measures, as applied in the HaploView software package (MJ Daly and JC Barrett, Whitehead Institute, MA, USA), was employed. A haplotype block was defined as a region in which all of the pairwise D' values exceeded 0.8. Blocks were not allowed to overlap.
The minimum number of SNPs that capture the maximum number of haplotypes (htSNPs)  were determined for each resulting block of each definition method. The htSNP2 programme (David Clayton, Cambridge, UK) implemented in Stata and the HaploView software package (MJ Daly and JC Barrett, Whitehead Institute, MA, USA), both making use of the EM algorithm, were employed to identify htSNPs. The r2 correlation measure, calculating the ability to predict frequencies at a series of loci using just the subset of htSNPs, was set to the stringent threshold of 0.95 for the htSNP2 programme. Good correspondence was observed between the two methods. HtSNPs were additionally identified with a set htSNP2 r2 threshold of 0.80, in order to check consistency under varying degrees of stringency.
Haplotype block characteristics according to different definition methods, applied to the total group of 137 SNPs.
Average block length (kb)
Average n SNPs/block
% of sequence covered
n SNPs in blocks (%)
Haplotype block characteristics according to different definition methods, applied to the subset of 100 common SNPs (minor allele frequency >0.2).
Average block length (kb)
Average n SNPs/block
% of sequence covered
n SNPs in blocks
Recently, numerous groups have studied the presence and distribution of haplotype blocks in the human genome, each proposing and utilising distinct block definition methods. Each study has examined different numbers of SNPs, dispersed throughout differently sized chromosomal regions, at varying minor allele frequency and map spacing, making use of diverse sample sizes [9, 11, 16, 25, 27]. The underlying design of this study reflects a realistic scenario, in which a region of several Mbs has been implicated in susceptibility to a human complex disease and is being refined through LD mapping. The ascertainment of SNPs through publicly available databases additionally represents practically favoured selection processes, giving rise to a well-recognised bias toward common polymorphisms and leading to, in this case, a median marker spacing of 15.5 kb (equivalent to that used by Dawson et al. 2002 ). This SNP density would be expected to give rise to apparently longer haplotype blocks compared to denser maps, such as the HapMap. The selection of unrelated individuals is in keeping with the current trend toward population-based, rather than family-based, studies and the pragmatic sample size of 378 chromosomes allows effective in silico haplotype inference.
The extent and variability of inter-marker LD in this study corroborates recent findings of distribution irregularity [6, 9, 13–15]. The observation that LD does not decay uniformly with physical distance exemplifies the need for haplotype block structure determination. Evidence for disequilibrium has been detected between SNPs as far as 1.7 Mb apart, extending much further than previously indicated through simulation . The discrepant LD patterns derived from distinct LD measures highlight the need for caution in interpreting and comparing studies, especially when using D', for which there is an upward bias with small sample sizes and rare allele frequencies.
Haplotype block definition methods employed in this study have been based both on measures of LD and on haplotype diversity, and applied to the same dataset, thus enabling direct comparison of their performance. Definitions 1  and 3  have been proposed in recent studies of block structure in human genomic regions. Definition 2, based on measures of LD, was developed as a simplified modification of Definition 1  to accommodate less stringent thresholds and criteria. Definition 5 was used to reflect block structure based on criteria setting a high threshold of D', but no length constraints. In addition, a novel diversity-based method was developed (Definition 4), which does not impose strict block boundaries and incorporates the notion of recombination events and recurrent mutation, factors known to diminish inter-marker LD.
All methods provided evidence for a block-like organisation of the genetic variation in the chromosomal region under investigation on 17q, characterised by marked differences among the distinct definitions, in accordance with previous observations [33, 34]. Overall, the haplotype diversity-based method (Definition 4) gave a more comprehensive coverage of the sequence, resulting in a higher number of blocks with a longer average physical size, compared to definitions based on measures of LD. These findings are in agreement with a recent study, comparing the performance of one LD-based with one haplotype-based block definition . Such differences in characterising the underlying genetic architecture of a region could have implications in the interpretation of association studies and the design of subsequent strategies. Inclusion of a greater proportion of the region into blocks maximises the chances that a significant association observed through a first scan will be encompassed within a haplotype block, thus delineating the interval on which further fine mapping attempts can be focused. Localisation of a positive result outside the boundaries of defined blocks would necessitate more intensive genotyping efforts targeted to the surrounding region. Although extended coverage of a sequence interval may prove useful, it could be artificial, stemming from methodological inadequacies, thereby leading to a false representation of the underlying genomic structure. Although the newly developed Definition 4 (n+1 method) resulted in higher sequence coverage, the lack of any LD constraints in this definition could lead to a falsely inflated detection of short haplotype blocks in cases of SNPs with rare minor allele frequencies. Of the LD-based methods, Definition 5 (D' high threshold method) provided the highest coverage of the sequence studied, although approximately 74% of the region did not fall into blocks. The observed inconsistency among methods illustrates the subjectivity of haplotype block definition and prevents the conclusive characterisation of the region's block structure.
Haplotype block assignment was found to change not only due to inter-method differences, but also as a result of altering parameters within the same set of definition criteria. These observations corroborate previous findings . Application of the same methods to the subset of common SNPs led to an overall reduction in the proportion of the sequence covered. Although marker spacing was sparser and the number of polymorphisms examined smaller, high minor allele frequency had an overwhelming effect on haplotype block size, generally resulting in shorter blocks. Definition 1 , however, appeared to be robust to such changes, therefore offering a possible mechanism to achieve consistency in block structure between SNP subgroups of varying allele frequencies. Among LD-based definitions, use of D' rather than r2 resulted in the generation of more haplotype blocks and in an increased coverage of the region. Similarly to when applied to the total set of markers, the newly introduced Definition 4 (n+1 method) produced the highest sequence coverage when examined in the subset of common SNPs only, although a proportion of relatively infrequent SNP pairs in low LD could have been falsely categorised as blocks.
Comparison of different haplotype block definitions in characterising the genomic organisation of the human chromosomal region 17q revealed discrepancies among methods and could, therefore, raise concerns about both the suitability of ad hoc approaches for the crude identification of block structure, as well as the validity of the notion of haplotype blocks as a genomic feature. However, the observed overlap in SNPs encompassed within blocks across all definitions used, indicated an underlying genetic architecture captured by all methods. Concordance among all definitions was additionally exhibited in calculating the subset of SNPs necessary to encapsulate the vast majority of genetic variation in the region. Selection of block definition method appeared to be irrelevant when genotyping a sample subset for all markers in order to identify haplotype tagging SNPs. In this study, the percentage of markers that needed to be typed was extremely high (over 90%), indicating that the marker map density employed was not suited to achieving significant cost-effectiveness through htSNP characterisation. Reassuringly, as all methods suggested typing the same number of markers, they probably also carry equal chances of detecting a possible association due to LD. The differences, however, would arise in interpreting downstream results and developing follow-up strategies.
The proposal of taking advantage of haplotype blocks to inform strategic designs in genetic association studies constitutes a welcome step forward, rather than a panacea, for the field of human complex disease genetics. In a realistic study design, the choice of block definition method could be of consequence in designing and interpreting genetic association scans. In addition, the inclusion of SNPs with rare minor allele frequencies appears to convolute, rather than clarify, the underlying genomic structure. Given the marker density of 15.5 kb, a whole genome scan by association would require approximately 100,000 SNPs to be genotyped. The findings of this study indicate that such a spacing would not be adequate for characterising the genomic architecture in sufficient detail through a haplotype block definition approach. Further issues inherent to the characterisation and utilisation of chromosomal underlying block structure need to be addressed in both real and simulated datasets, in order to clarify the settings in which haplotype blocks may prove useful.
We are grateful to Golden Helix Inc, for making the HelixTree™ software package available to us and to Neil Shephard for his valuable advice. This work was supported by funding from the MRC and arc, UK.
- Landegren U, Nilsson M, Kwok PY: Reading bits of genetic information: methods for single-nucleotide polymorphism analysis. Genome Res. 1998, 8: 769-776.PubMedGoogle Scholar
- Wang DG, Fan JB, Siao CJ, Berno A, Young P, Sapolsky R, Ghandour G, Perkins N, Winchester E, Spencer J, Kruglyak L, Stein L, Hsie L, Topaloglou T, Hubbell E, Robinson E, Mittmann M, Morris MS, Shen N, Kilburn D, Rioux J, Nusbaum C, Rozen S, Hudson TJ, Lander ES: Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome. Science. 1998, 280: 1077-1082. 10.1126/science.280.5366.1077.View ArticlePubMedGoogle Scholar
- Kruglyak L, Nickerson DA: Variation is the spice of life. Nat Genet. 2001, 27: 234-236. 10.1038/85776.View ArticlePubMedGoogle Scholar
- Cardon LR, Bell J: Association study designs for complex diseases. Nat Rev Genet. 2001, 2: 91-99. 10.1038/35052543.View ArticlePubMedGoogle Scholar
- Goldstein DB: Islands of linkage disequilibrium. Nat Genet. 2001, 29: 109-111. 10.1038/ng1001-109.View ArticlePubMedGoogle Scholar
- Reich DE, Cargill M, Bolk S, Ireland J, Sabeti PC, Richter DJ, Lavery T, Kouyoumjian R, Farhadian SF, Ward R, Lander ES: Linkage disequilibrium in the human genome. Nature. 2001, 411: 199-204. 10.1038/35075590.View ArticlePubMedGoogle Scholar
- Weale ME, Depondt C, Macdonald SJ, Smith A, Lai PS, Shorvon SD, Wood NW, Goldstein DB: Selection and evaluation of tagging SNPs in the neuronal-sodium-channel gene SCN1A: Implications for linkage disequilibrium gene mapping. Am J Hum Genet. 2003, 73: 551-565. 10.1086/378098.PubMed CentralView ArticlePubMedGoogle Scholar
- Makino C, Fujii Y, Kikuta R, Hirata N, Tani A, Shibata A, Ninomiya H, Tashiro N, Shibata H, Fukumaki Y: Positive association of the AMPA receptor subunit GluR4 gene (GRIA4) haplotype with schizophrenia: linkage disequilibrium mapping using SNPs evenly distributed across the gene region. Am J Med Genet B Neuropsychiatr Genet. 2003, 116: 17-22. 10.1002/ajmg.b.10041.View ArticleGoogle Scholar
- Dawson E, Abecasis GR, Bumpstead S, Chen Y, Hunt S, Beare DM, Pabial J, Dibling T, Tinsley E, Kirby S, Carter D, Papaspyridonos M, Livingstone S, Ganske R, Lohmussaar E, Zernant J, Tonisson N, Remm M, Magi R, Puurand T, Vilo J, Kurg A, Rice K, Deloukas P, Mott R, Metspalu A, Bentley DR, Cardon LR, Dunham I: A first-generation linkage disequilibrium map of human chromosome 22. Nature. 2002, 418: 544-548. 10.1038/nature00864.View ArticlePubMedGoogle Scholar
- Walsh EC, Mather KA, Schaffner SF, Farwell L, Daly MJ, Patterson N, Cullen M, Carrington M, Bugawan TL, Erlich H, Campbell J, Barrett J, Miller K, Thomson G, Lander ES, Rioux JD: An integrated haplotype map of the human major histocompatibility complex. Am J Hum Genet. 2003, 73: 580-590. 10.1086/378101.PubMed CentralView ArticlePubMedGoogle Scholar
- Patil N, Berno AJ, Hinds DA, Barrett WA, Doshi JM, Hacker CR, Kautzer CR, Lee DH, Marjoribanks C, McDonough DP, Nguyen BT, Norris MC, Sheehan JB, Shen N, Stern D, Stokowski RP, Thomas DJ, Trulson MO, Vyas KR, Frazer KA, Fodor SP, Cox DR: Blocks of limited haplotype diversity revealed by high-resolution scanning of human chromosome 21. Science. 2001, 294: 1719-1723. 10.1126/science.1065573.View ArticlePubMedGoogle Scholar
- Van Eerdewegh P, Little RD, Dupuis J, Del Mastro RG, Falls K, Simon J, Torrey D, Pandit S, McKenny J, Braunschweiger K, Walsh A, Liu Z, Hayward B, Folz C, Manning SP, Bawa A, Saracino L, Thackston M, Benchekroun Y, Capparell N, Wang M, Adair R, Feng Y, Dubois J, FitzGerald MG, Huang H, Gibson R, Allen KM, Pedan A, Danzig MR, Umland SP, Egan RW, Cuss FM, Rorke S, Clough JB, Holloway JW, Holgate ST, Keith TP: Association of the ADAM33 gene with bronchial hyperresponsiveness. Nature. 2002, 418: 426-430. 10.1038/nature00878.View ArticlePubMedGoogle Scholar
- Taillon-Miller P, Bauer-Sardina I, Saccone NL, Putzel J, Laitinen T, Cao A, Kere J, Pilia G, Rice JP, Kwok PY: Juxtaposed regions of extensive and minimal linkage disequilibrium in human Xq25 and Xq28. Nat Genet. 2000, 25: 324-328. 10.1038/77100.View ArticlePubMedGoogle Scholar
- Abecasis GR, Noguchi E, Heinzmann A, Traherne JA, Bhattacharyya S, Leaves NI, Anderson GG, Zhang Y, Lench NJ, Carey A, Cardon LR, Moffatt MF, Cookson WO: Extent and distribution of linkage disequilibrium in three genomic regions. Am J Hum Genet. 2001, 68: 191-197. 10.1086/316944.PubMed CentralView ArticlePubMedGoogle Scholar
- Pritchard JK, Przeworski M: Linkage disequilibrium in humans: models and data. Am J Hum Genet. 2001, 69: 1-14. 10.1086/321275.PubMed CentralView ArticlePubMedGoogle Scholar
- Gabriel SB, Schaffner SF, Nguyen H, Moore JM, Roy J, Blumenstiel B, Higgins J, DeFelice M, Lochner A, Faggart M, Liu-Cordero SN, Rotimi C, Adeyemo A, Cooper R, Ward R, Lander ES, Daly MJ, Altshuler D: The structure of haplotype blocks in the human genome. Science. 2002, 296: 2225-2229. 10.1126/science.1069424.View ArticlePubMedGoogle Scholar
- Stumpf MPH: Haplotype diversity and the block structure of linkage disequilibrium. Trends Genet. 2002, 18: 226-228. 10.1016/S0168-9525(02)02641-0.View ArticlePubMedGoogle Scholar
- Kauppi L, Sajantila A, Jeffreys AJ: Recombination hotspots rather than population history dominate linkage disequilibrium in the MHC class II region. Hum Mol Genet. 2003, 12: 33-40. 10.1093/hmg/ddg008.View ArticlePubMedGoogle Scholar
- Lai E, Bowman C, Bansal A, Hughes A, Mosteller M, Roses AD: Medical applications of haplotype-based SNP maps: learning to walk before we run. Nat Genet. 2002, 32: 353-10.1038/ng1102-353.View ArticlePubMedGoogle Scholar
- Weiss KM, Clark AG: Linkage disequilibrium and the mapping of complex human traits. Trends Genet. 2002, 18: 19-24. 10.1016/S0168-9525(01)02550-1.View ArticlePubMedGoogle Scholar
- Zhang K, Calabrese P, Nordborg M, Sun F: Haplotype block structure and its applications to association studies: power and study designs. Am J Hum Genet. 2002, 71: 1386-1394. 10.1086/344780.PubMed CentralView ArticlePubMedGoogle Scholar
- Omi K, Ohashi J, Patarapotikul J, Hananantachai H, Naka I, Looareesuwan S, Tokunaga K: CD36 polymorphism is associated with protection from cerebral malaria. Am J Hum Genet. 2003, 72: 364-374. 10.1086/346091.PubMed CentralView ArticlePubMedGoogle Scholar
- Cardon LR, Abecasis GR: Using haplotype blocks to map human complex trait loci. Trends Genet. 2003, 19: 135-140. 10.1016/S0168-9525(03)00022-2.View ArticlePubMedGoogle Scholar
- Wang N, Akey JM, Zhang K, Chakraborty K, Jin L: Distribution of recombination crossovers and the origin of haplotype blocks: the interplay of population history, recombination, and mutation. Am J Hum Genet. 2002, 71: 1227-1234. 10.1086/344398.PubMed CentralView ArticlePubMedGoogle Scholar
- Daly MJ, Rioux JD, Schaffner SF, Hudson TJ, Lander ES: High-resolution haplotype structure in the human genome. Nat Genet. 2001, 29: 229-232. 10.1038/ng1001-229.View ArticlePubMedGoogle Scholar
- Zhang K, Deng M, Chen T, Waterman MS, Sun F: A dynamic programming algorithm for haplotype block partitioning. PNAS. 2002, 99: 7335-7339. 10.1073/pnas.102186799.PubMed CentralView ArticlePubMedGoogle Scholar
- Phillips MS, Lawrence R, Sachidanandam R, Morris AP, Balding DJ, Donaldson MA, Studebaker JF, Ankener WM, Alfisi SV, Kuo FS, Camisa AL, Pazorov V, Scott KE, Carey BJ, Faith J, Katari G, Bhatti HA, Cyr JM, Derohannessian V, Elosua C, Forman AM, Grecco NM, Hock CR, Kuebler JM, Lathrop JA, Mockler MA, Nachtman EP, Restine SL, Varde SA, Hozza MJ, Gelfand CA, Broxholme J, Abecasis GR, Boyce-Jacino MT, Cardon LR: Chromosome-wide distribution of haplotype blocks and the role of recombination hot spots. Nat Genet. 2003, 33: 382-387. 10.1038/ng1100.View ArticlePubMedGoogle Scholar
- Johnson GC, Esposito L, Barratt BJ, Smith AN, Heward J, Di Genova G, Ueda H, Cordell HJ, Eaves IA, Dudbridge F, Twells RC, Payne F, Hughes W, Nutland S, Stevens H, Carr P, Tuomilehto-Wolf E, Tuomilehto J, Gough SC, Clayton DG, Todd JA: Haplotype tagging for the identification of common disease genes. Nat Genet. 2001, 29: 233-237. 10.1038/ng1001-233.View ArticlePubMedGoogle Scholar
- Reich DE, Gabriel SB, Altshuler D: Quality and completeness of SNP databases. Nat Genet. 2003, 33: 457-458. 10.1038/ng1133.View ArticlePubMedGoogle Scholar
- Kruglyak L: Prospects for whole-genome linkage disequilibrium mapping of common disease genes. Nat Genet. 1999, 22: 139-144. 10.1038/9642.View ArticlePubMedGoogle Scholar
- Schwartz R, Halldorsson BV, Bafna V, Clark AG, Istrail S: Robustness of inference of haplotype block structure. J Comput Biol. 2003, 10: 13-19. 10.1089/106652703763255642.View ArticlePubMedGoogle Scholar
- Schulze TG, Zhang K, Chen Y-S, Akula N, Sun F, McMahon FJ: Defining haplotype blocks and tag single-nucleotide polymorphisms in the human genome. Hum Mol Genet. 2004, 13: 335-42. 10.1093/hmg/ddh035.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.