Table 1 Sequences of primers for gene fragment isolation and PCR results
From: High resolution physical mapping of single gene fragments on pachytene chromosome 4 and 7 of Rosa
Gene | Abbreviation | Primers, 5′-3′ | Location on Fragaria pseudo-chromosomes | Expected length PCR product (bp) | Length obtained PCR products (bp) |
|---|---|---|---|---|---|
MLO-like protein | MLO3 | F: AAAACACCAACATGGGCAGT | FvChr 7: 15397809..15393519 | 1675 | 1700 |
R: TTCCGAAAATCAAAGGTCGT | |||||
MLO-like protein | MLO2 | F: AGGATTTCAAGGTCGTGGTG | FvChr6: 34503533..34507423 | 1852 | 1800 |
R: TGGTCGGCTAGCATTTTTCT | |||||
ATPase | AAA-2 | F: GTTCCCTTTGTCATTGCAG | FvChr7: 21485846.. 21481090 | 2718 | 3400 |
R: ACGGCCTCTTCATCAATT | |||||
Ubiquitin protein ligase | RIN-2 | F: TCCTTCAGCTACACCATTGAC | FvChr7: 19866961..19871497 | 2228 | 2500 |
R: AAATTGCGCGTTCCTACT | |||||
Monodehydroascorbate reductase | MDAR | F: GAGGCGGTATGGTTAATTT | FvChr6: 12864594..12867898 | 2417 | 2800 |
R: AAACTTGGGCTTTGGTGA | |||||
Villin-2-like | Villin | F: CTCGCTTCTTCACAACATACT | FvChr6: 33309900..33321407 | 3851 | 900 |
R:TTCACTGCCATTTTCATCCT | |||||
Mannosylglycoprotein endo-beta-mannosidase | MGM | F: CGGCATGGAAAATGAGTCAA | FvChr6: 5180627..5186123 | 3017 | 3000 |
R: GAACAAAGGGATCTGCCA | |||||
Phenylalanine ammonia lyasea | PAL | F: ACCACTGGKTTTGGTGCWAC | FvChr6:34874086–34877587 | - | 1700 |
R: CCYTTGAASCCATAATCCAA | |||||
Pyrroline-5-Carboxylate Synthasea | P5CS | F: GCTGGCATCCCTGTTGTTAT | FvChr7: 17624431–17630820 | - | 1700 |
R: CTTCGGATCGCTAATGAAGC |